TY - JOUR
T1 - Characterization of potential probiotic lactic acid bacteria isolated from camel milk
AU - Abushelaibi, Aisha
AU - Al-Mahadin, Suheir
AU - El-Tarabily, Khaled
AU - Shah, Nagendra P.
AU - Ayyash, Mutamed
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/6/1
Y1 - 2017/6/1
N2 - The aim of this study was to investigate probiotic characteristics and fermentation profile of selected lactic acid bacteria isolated from raw camel milk. Physiological properties, cell surface properties (hydrophobicity, autoaggregation, co-aggregation), acid and bile tolerance, bile salt hydrolysis, cholesterol removing, exopolysaccharide (EPS) production, hemolytic and antimicrobial activities, resistance toward lysozyme and six antibiotics, and fermentation profile (growth, pH, and proteolysis) were examined. 16S rRNA sequencing was carried out to identify six presumptive LAB isolates. In general, all identified LAB (Lactococcus lactis KX881768, Lactobacillus plantarum KX881772, Lactococcus lactis KX881782 and Lactobacillus plantarum KX881779) showed auto-aggregation ability, high cholesterol removal ability, high co-aggregation, strong antimicrobial activity and EPS production. Among the isolates, Lactococcus lactis KX881768, Lactobacillus plantarum KX881772, Lactococcus lactis KX881782 and Lactobacillus plantarum KX881779 exhibited remarkable cholesterol removal abilities. Similarly, Lactobacillus plantarum KX881779, and Lactococcus lactis KX881782 showed very promising fermentation profiles.
AB - The aim of this study was to investigate probiotic characteristics and fermentation profile of selected lactic acid bacteria isolated from raw camel milk. Physiological properties, cell surface properties (hydrophobicity, autoaggregation, co-aggregation), acid and bile tolerance, bile salt hydrolysis, cholesterol removing, exopolysaccharide (EPS) production, hemolytic and antimicrobial activities, resistance toward lysozyme and six antibiotics, and fermentation profile (growth, pH, and proteolysis) were examined. 16S rRNA sequencing was carried out to identify six presumptive LAB isolates. In general, all identified LAB (Lactococcus lactis KX881768, Lactobacillus plantarum KX881772, Lactococcus lactis KX881782 and Lactobacillus plantarum KX881779) showed auto-aggregation ability, high cholesterol removal ability, high co-aggregation, strong antimicrobial activity and EPS production. Among the isolates, Lactococcus lactis KX881768, Lactobacillus plantarum KX881772, Lactococcus lactis KX881782 and Lactobacillus plantarum KX881779 exhibited remarkable cholesterol removal abilities. Similarly, Lactobacillus plantarum KX881779, and Lactococcus lactis KX881782 showed very promising fermentation profiles.
KW - Camel milk
KW - Cholesterol removal
KW - Lactic acid bacteria
KW - Lactobacili
KW - Probiotic
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U2 - 10.1016/j.lwt.2017.01.041
DO - 10.1016/j.lwt.2017.01.041
M3 - Article
AN - SCOPUS:85010402363
SN - 0023-6438
VL - 79
SP - 316
EP - 325
JO - LWT - Food Science and Technology
JF - LWT - Food Science and Technology
ER -