Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae

J. Michael Conlon; Manju Prajeep; Milena Mechkarska; Laurent Coquet; Jérôme Leprince; Thierry Jouenne; Hubert Vaudry; Jay D. King

doi:10.1016/j.cbd.2013.10.002

Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae

J. Michael Conlon
, Manju Prajeep
, Milena Mechkarska
, Laurent Coquet
, Jérôme Leprince
, Thierry Jouenne
, Hubert Vaudry
, Jay D. King

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The family Pipidae comprises the genera Hymenochirus, Pipa, Pseudhymenochirus, Silurana, and Xenopus but phylogenetic relationships within the family are unclear. Peptidomic analysis of norepinephrine-stimulated skin secretions from Pseudhymenochirus merlini Chabanaud, 1920, the single species within the genus Pseudhymenochirus, led to identification of 13 host-defense peptides with antimicrobial activity. Two peptides (hymenochirin-1Pa and -1Pb) show structural similarity to hymenochirin-1B from Hymenochirus boettgeri and eight peptides (hymenochirin-5Pa, -5Pb, -5Pc, -5Pd, -5Pe, -5Pf, 5Pg and -5Ph) are structurally similar to each other and to hymenochirin-5B from H. boettgeri. Two peptides differing by a single amino acid (IKIPSFFRNILKKVGKEAVSLM/I AGALKQS), termed pseudhymenochirin-1Pa and -1Pb, and pseudhymenochirin-2Pa (GIFPIFAKLLGKVIKVASSLISKGRTE) do not resemble host-defense peptides previously isolated from pipid frogs. Hymenochirin-5Pe was the most abundant peptide in the secretions and hymenochirin-1Pa the most potent against Staphylococcus aureus (MIC = 2.5 μM) and Escherichia coli (MIC = 10 μM). The data support a close phylogenetic relationship between Hymenochirus and Pseudhymenochirus that is distinct from the Xenopodinae (Xenopus + Silurana) clade with Pipa sister-group to all other extant pipids.

Original language	English
Pages (from-to)	352-357
Number of pages	6
Journal	Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume	8
Issue number	4
DOIs	https://doi.org/10.1016/j.cbd.2013.10.002
Publication status	Published - 2013

Keywords

Antimicrobial peptide
Frog skin
Hymenochirus
Pipidae
Pseudhymenochirus

ASJC Scopus subject areas

Biochemistry
Physiology
Aquatic Science
Animal Science and Zoology
Molecular Biology
Genetics

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10.1016/j.cbd.2013.10.002

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Conlon, J. M., Prajeep, M., Mechkarska, M., Coquet, L., Leprince, J., Jouenne, T., Vaudry, H., & King, J. D. (2013). Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 8(4), 352-357. https://doi.org/10.1016/j.cbd.2013.10.002

Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae. / Conlon, J. Michael; Prajeep, Manju; Mechkarska, Milena et al.
In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 8, No. 4, 2013, p. 352-357.

Research output: Contribution to journal › Article › peer-review

Conlon, JM, Prajeep, M, Mechkarska, M, Coquet, L, Leprince, J, Jouenne, T, Vaudry, H & King, JD 2013, 'Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae', Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, vol. 8, no. 4, pp. 352-357. https://doi.org/10.1016/j.cbd.2013.10.002

Conlon JM, Prajeep M, Mechkarska M, Coquet L, Leprince J, Jouenne T et al. Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 2013;8(4):352-357. doi: 10.1016/j.cbd.2013.10.002

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title = "Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae",

abstract = "The family Pipidae comprises the genera Hymenochirus, Pipa, Pseudhymenochirus, Silurana, and Xenopus but phylogenetic relationships within the family are unclear. Peptidomic analysis of norepinephrine-stimulated skin secretions from Pseudhymenochirus merlini Chabanaud, 1920, the single species within the genus Pseudhymenochirus, led to identification of 13 host-defense peptides with antimicrobial activity. Two peptides (hymenochirin-1Pa and -1Pb) show structural similarity to hymenochirin-1B from Hymenochirus boettgeri and eight peptides (hymenochirin-5Pa, -5Pb, -5Pc, -5Pd, -5Pe, -5Pf, 5Pg and -5Ph) are structurally similar to each other and to hymenochirin-5B from H. boettgeri. Two peptides differing by a single amino acid (IKIPSFFRNILKKVGKEAVSLM/I AGALKQS), termed pseudhymenochirin-1Pa and -1Pb, and pseudhymenochirin-2Pa (GIFPIFAKLLGKVIKVASSLISKGRTE) do not resemble host-defense peptides previously isolated from pipid frogs. Hymenochirin-5Pe was the most abundant peptide in the secretions and hymenochirin-1Pa the most potent against Staphylococcus aureus (MIC = 2.5 μM) and Escherichia coli (MIC = 10 μM). The data support a close phylogenetic relationship between Hymenochirus and Pseudhymenochirus that is distinct from the Xenopodinae (Xenopus + Silurana) clade with Pipa sister-group to all other extant pipids.",

keywords = "Antimicrobial peptide, Frog skin, Hymenochirus, Pipidae, Pseudhymenochirus",

author = "Conlon, \{J. Michael\} and Manju Prajeep and Milena Mechkarska and Laurent Coquet and J{\'e}r{\^o}me Leprince and Thierry Jouenne and Hubert Vaudry and King, \{Jay D.\}",

note = "Funding Information: This work was supported by the Terry Fox Fund for Cancer Research , a Faculty Support Grant and a University Research Grant from U.A.E. University . The authors thank No{\'e}mio Sousa for supplying the captive-bred frogs.",

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doi = "10.1016/j.cbd.2013.10.002",

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T2 - Insights into phylogenetic relationships among the Pipidae

AU - Conlon, J. Michael

AU - Prajeep, Manju

AU - Mechkarska, Milena

AU - Coquet, Laurent

AU - Leprince, Jérôme

AU - Jouenne, Thierry

AU - Vaudry, Hubert

AU - King, Jay D.

N1 - Funding Information: This work was supported by the Terry Fox Fund for Cancer Research , a Faculty Support Grant and a University Research Grant from U.A.E. University . The authors thank Noémio Sousa for supplying the captive-bred frogs.

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AB - The family Pipidae comprises the genera Hymenochirus, Pipa, Pseudhymenochirus, Silurana, and Xenopus but phylogenetic relationships within the family are unclear. Peptidomic analysis of norepinephrine-stimulated skin secretions from Pseudhymenochirus merlini Chabanaud, 1920, the single species within the genus Pseudhymenochirus, led to identification of 13 host-defense peptides with antimicrobial activity. Two peptides (hymenochirin-1Pa and -1Pb) show structural similarity to hymenochirin-1B from Hymenochirus boettgeri and eight peptides (hymenochirin-5Pa, -5Pb, -5Pc, -5Pd, -5Pe, -5Pf, 5Pg and -5Ph) are structurally similar to each other and to hymenochirin-5B from H. boettgeri. Two peptides differing by a single amino acid (IKIPSFFRNILKKVGKEAVSLM/I AGALKQS), termed pseudhymenochirin-1Pa and -1Pb, and pseudhymenochirin-2Pa (GIFPIFAKLLGKVIKVASSLISKGRTE) do not resemble host-defense peptides previously isolated from pipid frogs. Hymenochirin-5Pe was the most abundant peptide in the secretions and hymenochirin-1Pa the most potent against Staphylococcus aureus (MIC = 2.5 μM) and Escherichia coli (MIC = 10 μM). The data support a close phylogenetic relationship between Hymenochirus and Pseudhymenochirus that is distinct from the Xenopodinae (Xenopus + Silurana) clade with Pipa sister-group to all other extant pipids.

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Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae

Abstract

Keywords

ASJC Scopus subject areas

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