TY - JOUR
T1 - Characterization of the subunits in an apparently homogeneous subpopulation of Clostridium thermocellum cellulosomes
AU - Ali, Bassam R.S.
AU - Romaniec, Marek P.M.
AU - Hazlewood, Geoffrey P.
AU - Freedman, Robert B.
N1 - Funding Information:
This work was supportedb y a grant from the Biotechnology and Biological Sciences Research Council (BBRSC). The authorst hank Steve Cross, Kanokwan Poomputsa,a nd Antonio Ciruela for helpful discussions and technical assistance.
PY - 1995/8
Y1 - 1995/8
N2 - Clostridium thermocellum cellulosomes isolated by cellulose affinity chromatography were fractionated by anion exchange chromatography into apparently homogeneous subpopulations that differed with respect to enzyme activity and subunit composition. One such subpopulation contained predominantly six subunits and was closely similar to the "subcellulosome" described by Kobayashi et al. (Kobayashi, T., Romaniec, M. P. M., Fauth, U., and Demain, A. L., Appl. Environ. Microbiol., 1990, 56, 3040-3046). Avicelase specific activity of this homogeneous subpopulation was slightly higher than that of unfractionated cellulosomes, but the two preparations were similarly affected by Ca2+, dithiothreitol, and cellobiose. Determination of their N-terminal sequences and enzyme activities has enabled three of the six major subunits of the subpopulation of cellulosomes to be positively identified as known components of the C. thermocellum cellulase complex; the other three subunits did not match up with previously characterized cellulosomal proteins.
AB - Clostridium thermocellum cellulosomes isolated by cellulose affinity chromatography were fractionated by anion exchange chromatography into apparently homogeneous subpopulations that differed with respect to enzyme activity and subunit composition. One such subpopulation contained predominantly six subunits and was closely similar to the "subcellulosome" described by Kobayashi et al. (Kobayashi, T., Romaniec, M. P. M., Fauth, U., and Demain, A. L., Appl. Environ. Microbiol., 1990, 56, 3040-3046). Avicelase specific activity of this homogeneous subpopulation was slightly higher than that of unfractionated cellulosomes, but the two preparations were similarly affected by Ca2+, dithiothreitol, and cellobiose. Determination of their N-terminal sequences and enzyme activities has enabled three of the six major subunits of the subpopulation of cellulosomes to be positively identified as known components of the C. thermocellum cellulase complex; the other three subunits did not match up with previously characterized cellulosomal proteins.
KW - Clostridium thermocellum
KW - cellulose hydrolysis
KW - cellulosomes
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U2 - 10.1016/0141-0229(94)00118-B
DO - 10.1016/0141-0229(94)00118-B
M3 - Article
C2 - 7646877
AN - SCOPUS:0029089678
SN - 0141-0229
VL - 17
SP - 705
EP - 711
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 8
ER -