TY - JOUR
T1 - Characterization of virulence marker antigen of Shigella spp. and enteroinvasive Escherichia coli
AU - Pal, T.
AU - Formal, S. B.
AU - Hale, T. L.
PY - 1989
Y1 - 1989
N2 - Antisera produced in rabbits immunized with an enteroinvasive O143 strain of Escherichia coli were absorbed with an avirulent derivative of the same strain. The resulting sera have been previously shown to recognize enteroinvasive pathogens when used in an enzyme-linked immunosorbent assay. In the current study, Western blots (immunoblots) showed that such an absorbed rabbit antiserum recognized two proteins (IpaB and IpaC) which are encoded by a large, virulence-associated plasmid. These proteins are the apparent constituents of the virulence marker antigen which is expressed by shigellae and enteroinvasive E. coli.
AB - Antisera produced in rabbits immunized with an enteroinvasive O143 strain of Escherichia coli were absorbed with an avirulent derivative of the same strain. The resulting sera have been previously shown to recognize enteroinvasive pathogens when used in an enzyme-linked immunosorbent assay. In the current study, Western blots (immunoblots) showed that such an absorbed rabbit antiserum recognized two proteins (IpaB and IpaC) which are encoded by a large, virulence-associated plasmid. These proteins are the apparent constituents of the virulence marker antigen which is expressed by shigellae and enteroinvasive E. coli.
UR - http://www.scopus.com/inward/record.url?scp=0024536339&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024536339&partnerID=8YFLogxK
U2 - 10.1128/jcm.27.3.561-563.1989
DO - 10.1128/jcm.27.3.561-563.1989
M3 - Article
C2 - 2654184
AN - SCOPUS:0024536339
SN - 0095-1137
VL - 27
SP - 561
EP - 563
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 3
ER -