Abstract
Galanin was measured by radioimmunoassay in extracts of pig, cat and rat adrenals using non-C- and mid to C-terminally directed antibodies. The extracts were fractioned by gel chromatography and HPLC. The non-C-terminal galanin immunoreactivity in pig was 92.8 ± 11.7 pmol g, in cat 9.1 ± 0.9 pmol g and in rat < 1 pmol g. Two higher molecular forms of galanin have been identified in both pig and cat adrenal. One major large form behaves as if it was N-terminally extended (Kav, pig 0.58, cat 0.48) and the other, a very high-molecular-mass form (Kav pig 0.10, 0.24, cat 0.10), as if it had both N- and C-terminal extensions. Galanin Molecular form Adrenal gland Radioimmunoassay Chromatography.
Original language | English |
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Pages (from-to) | 327-331 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 201 |
Issue number | 2 |
DOIs | |
Publication status | Published - Jun 9 1986 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology