TY - JOUR
T1 - Chromatographic evidence for high-molecular-mass galanin immunoreactivity in pig and cat adrenal glands
AU - Bauer, Friedrich E.
AU - Adrian, Thomas E.
AU - Yanaihara, Noboru
AU - Polak, Julia M.
AU - Bloom, Stephen R.
PY - 1986/6/9
Y1 - 1986/6/9
N2 - Galanin was measured by radioimmunoassay in extracts of pig, cat and rat adrenals using non-C- and mid to C-terminally directed antibodies. The extracts were fractioned by gel chromatography and HPLC. The non-C-terminal galanin immunoreactivity in pig was 92.8 ± 11.7 pmol g, in cat 9.1 ± 0.9 pmol g and in rat < 1 pmol g. Two higher molecular forms of galanin have been identified in both pig and cat adrenal. One major large form behaves as if it was N-terminally extended (Kav, pig 0.58, cat 0.48) and the other, a very high-molecular-mass form (Kav pig 0.10, 0.24, cat 0.10), as if it had both N- and C-terminal extensions. Galanin Molecular form Adrenal gland Radioimmunoassay Chromatography.
AB - Galanin was measured by radioimmunoassay in extracts of pig, cat and rat adrenals using non-C- and mid to C-terminally directed antibodies. The extracts were fractioned by gel chromatography and HPLC. The non-C-terminal galanin immunoreactivity in pig was 92.8 ± 11.7 pmol g, in cat 9.1 ± 0.9 pmol g and in rat < 1 pmol g. Two higher molecular forms of galanin have been identified in both pig and cat adrenal. One major large form behaves as if it was N-terminally extended (Kav, pig 0.58, cat 0.48) and the other, a very high-molecular-mass form (Kav pig 0.10, 0.24, cat 0.10), as if it had both N- and C-terminal extensions. Galanin Molecular form Adrenal gland Radioimmunoassay Chromatography.
UR - http://www.scopus.com/inward/record.url?scp=0022536073&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022536073&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(86)80633-0
DO - 10.1016/0014-5793(86)80633-0
M3 - Article
C2 - 2423373
AN - SCOPUS:0022536073
VL - 201
SP - 327
EP - 331
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -