Chromatographic evidence for high-molecular-mass galanin immunoreactivity in pig and cat adrenal glands

Galanin was measured by radioimmunoassay in extracts of pig, cat and rat adrenals using non-C- and mid to C-terminally directed antibodies. The extracts were fractioned by gel chromatography and HPLC. The non-C-terminal galanin immunoreactivity in pig was 92.8 ± 11.7 pmol g, in cat 9.1 ± 0.9 pmol g and in rat < 1 pmol g. Two higher molecular forms of galanin have been identified in both pig and cat adrenal. One major large form behaves as if it was N-terminally extended (K_av, pig 0.58, cat 0.48) and the other, a very high-molecular-mass form (K_av pig 0.10, 0.24, cat 0.10), as if it had both N- and C-terminal extensions. Galanin Molecular form Adrenal gland Radioimmunoassay Chromatography.