Cofactor engineering for enhancing the flux of metabolic pathways

M. Kalim Akhtar, Patrik R. Jones

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

The manufacture of a diverse array of chemicals is now possible with biologically engineered strains, an approach that is greatly facilitated by the emergence of synthetic biology. This is principally achieved through pathway engineering in which enzyme activities are coordinated within a genetically amenable host to generate the product of interest. A great deal of attention is typically given to the quantitative levels of the enzymes with little regard to their overall qualitative states. This highly constrained approach fails to consider other factors that may be necessary for enzyme functionality. In particular, enzymes with physically bound cofactors, otherwise known as holoenzymes, require careful evaluation. Herein, we discuss the importance of cofactors for biocatalytic processes and show with empirical examples why the synthesis and integration of cofactors for the formation of holoenzymes warrant a great deal of attention within the context of pathway engineering.

Original languageEnglish
Article number30
JournalFrontiers in Bioengineering and Biotechnology
Volume2
Issue numberSEP
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Cofactors
  • Enzymatic activity
  • Fe-S clusters
  • Metabolic pathway engineering
  • Synthetic biology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

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