TY - JOUR
T1 - Colorimetric and visual dopamine assay based on the use of gold nanorods
AU - Teo, Peik See
AU - Rameshkumar, Perumal
AU - Pandikumar, Alagarsamy
AU - Jiang, Zhong Tao
AU - Altarawneh, Mohammednoor
AU - Huang, Nay Ming
N1 - Publisher Copyright:
© 2017, Springer-Verlag GmbH Austria.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - The authors describe an optical method for the determination of dopamine (DA). It is based on the use of gold nanorods (AuNRs) with an aspect ratio of 3.2 and an absorption maximum at 700 nm. The AuNRs were synthesized via a seed-mediated growth route. The addition of various DA concentrations to the aqueous AuNRs results in a color change bluish-purple to colorless. The addition of DA is accompanied by a linear decrease in absorbance, a blue-shift of the peak to 687 nm, and the formation of a new band peaking at 720 nm on increasing in the DA concentration to 100 μM. It is found that DA causes an aggregation of the AuNRs through the formation of interparticle plasmon-linkages between DA molecules and AuNRs. This was confirmed by transmission electron microscopy. This new colorimetric probe for DA shows good selectivity for DA even in the presence of potentially interfering species. Several linearranges are observed between the changes in absorption at around 687 and at around 720 nm and the DA concentration in the range between 100 nM and 10 mM. The limit of detection is 30 nM. The method was successfully employed to the determination of DA in spiked human urine samples.
AB - The authors describe an optical method for the determination of dopamine (DA). It is based on the use of gold nanorods (AuNRs) with an aspect ratio of 3.2 and an absorption maximum at 700 nm. The AuNRs were synthesized via a seed-mediated growth route. The addition of various DA concentrations to the aqueous AuNRs results in a color change bluish-purple to colorless. The addition of DA is accompanied by a linear decrease in absorbance, a blue-shift of the peak to 687 nm, and the formation of a new band peaking at 720 nm on increasing in the DA concentration to 100 μM. It is found that DA causes an aggregation of the AuNRs through the formation of interparticle plasmon-linkages between DA molecules and AuNRs. This was confirmed by transmission electron microscopy. This new colorimetric probe for DA shows good selectivity for DA even in the presence of potentially interfering species. Several linearranges are observed between the changes in absorption at around 687 and at around 720 nm and the DA concentration in the range between 100 nM and 10 mM. The limit of detection is 30 nM. The method was successfully employed to the determination of DA in spiked human urine samples.
KW - Interplasmon coupling
KW - Localized surface plasmon resonance
KW - Neurotransmitter
KW - Optical determination
KW - Recovery analysis
KW - Transmission electron microscopy
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U2 - 10.1007/s00604-017-2435-5
DO - 10.1007/s00604-017-2435-5
M3 - Article
AN - SCOPUS:85028563520
SN - 0026-3672
VL - 184
SP - 4125
EP - 4132
JO - Mikrochimica Acta
JF - Mikrochimica Acta
IS - 10
ER -