TY - JOUR
T1 - Combined estrogen and ghrelin administration decreases expression of p27kip1 and proportion of isomyosin type I in the striated urethral and anal sphincters and levator ani of old ovariectomized rats
AU - Rizk, Diaa E.E.
AU - Hassan, Hazem A.
AU - Al-Marzouqi, Ahmed H.
AU - Shafiullah, Mohammed
AU - Fahim, Mohamed A.
N1 - Funding Information:
Acknowledgments This study was supported by a research grant from the Research Affairs, United Arab Emirates University, N 01-01-8-12/04. We would like to thank Dr. S. Benedict for technical assistance with radioimmunoassay of serum growth hormone.
PY - 2008
Y1 - 2008
N2 - We compared estrogen and/or ghrelin effects on pelvic floor muscles in old versus young adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n= 24 × 2) received 42 daily intraperitoneal 17-β estradiol (10 μg kg-1), ghrelin (2 μg kg-1), both, or vehicle (n = 6 × 4/group). Cytoplasmic p27kip1 expression and isomyosin I proportion in striated urethral and anal sphincters and levator ani were measured, respectively, by Western blot analysis and gel electrophoresis with immunohistochemistry of muscle ghrelin receptors and radioimmunoassay of circulating growth hormone. In young adult rats, estrogen significantly decreased cytoplasmic p27kip1 and isomyosin I signal intensities. In old rats, ghrelin and estrogen/ghrelin significantly decreased both intensities with greater estrogen/ghrelin effect. Ghrelin receptors were not immunostained in any muscle. Estrogen and/or ghrelin significantly increased or decreased, respectively, circulating growth hormone in old and young adult rats. Estrogen/ghrelin administration reversed pelvic floor muscle ageing changes in old ovariectomized rats through growth hormone production.
AB - We compared estrogen and/or ghrelin effects on pelvic floor muscles in old versus young adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n= 24 × 2) received 42 daily intraperitoneal 17-β estradiol (10 μg kg-1), ghrelin (2 μg kg-1), both, or vehicle (n = 6 × 4/group). Cytoplasmic p27kip1 expression and isomyosin I proportion in striated urethral and anal sphincters and levator ani were measured, respectively, by Western blot analysis and gel electrophoresis with immunohistochemistry of muscle ghrelin receptors and radioimmunoassay of circulating growth hormone. In young adult rats, estrogen significantly decreased cytoplasmic p27kip1 and isomyosin I signal intensities. In old rats, ghrelin and estrogen/ghrelin significantly decreased both intensities with greater estrogen/ghrelin effect. Ghrelin receptors were not immunostained in any muscle. Estrogen and/or ghrelin significantly increased or decreased, respectively, circulating growth hormone in old and young adult rats. Estrogen/ghrelin administration reversed pelvic floor muscle ageing changes in old ovariectomized rats through growth hormone production.
KW - Ageing
KW - Ghrelin
KW - Isomyosin
KW - Menopause
KW - Rat
KW - p27
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U2 - 10.1007/s00192-008-0638-3
DO - 10.1007/s00192-008-0638-3
M3 - Article
C2 - 18496635
AN - SCOPUS:51949090845
SN - 0937-3462
VL - 19
SP - 1363
EP - 1369
JO - International Urogynecology Journal
JF - International Urogynecology Journal
IS - 10
ER -