Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats

Diaa E.E. Rizk, Hazem A. Hassan, Ahmed H. Al-Marzouqi, Gaber A. Ramadan, Soha S. Al-Kedrah, Sayel A. Daoud, Mohamed A. Fahim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

We compare the effects of estrogen and/or ghrelin on vascular counts and collagen I/III ratio of urethral and anal canal submucosa in old vs young-adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n=24×2) received 42 daily intraperitoneal 17-β estradiol (10 μg/kg), ghrelin (2 μg/kg), both, or vehicle (n=6×4 per group). Blood vessel counts and collagen I/III ratio were measured, respectively, by light microscopy and Western blot analysis with immunohistochemistry of ghrelin receptors. Estrogen significantly increased urethral and anal vascular counts and collagen I/III ratio in young-adult rats. In old rats, only combined estrogen/ghrelin administration significantly increased both variables. This was not observed with estrogen or ghrelin separately. Ghrelin receptors were immunostained in urethral and anal submucosa of all samples. Combined estrogen/ghrelin administration restored postovariectomy urethral and anal canal submucosal vessel number and collagen I/III ratio in old rats suggesting independent ageing effect.

Original languageEnglish
Pages (from-to)547-552
Number of pages6
JournalInternational Urogynecology Journal
Volume19
Issue number4
DOIs
Publication statusPublished - Apr 2008

Keywords

  • Ageing
  • Estrogen
  • Ghrelin
  • Incontinence
  • Menopause
  • Rat

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Urology

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