Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats

Diaa E.E. Rizk; Hazem A. Hassan; Ahmed H. Al-Marzouqi; Gaber A. Ramadan; Soha S. Al-Kedrah; Sayel A. Daoud; Mohamed A. Fahim

doi:10.1007/s00192-007-0462-1

Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats

Diaa E.E. Rizk, Hazem A. Hassan, Ahmed H. Al-Marzouqi, Gaber A. Ramadan, Soha S. Al-Kedrah, Sayel A. Daoud, Mohamed A. Fahim

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We compare the effects of estrogen and/or ghrelin on vascular counts and collagen I/III ratio of urethral and anal canal submucosa in old vs young-adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n=24×2) received 42 daily intraperitoneal 17-β estradiol (10 μg/kg), ghrelin (2 μg/kg), both, or vehicle (n=6×4 per group). Blood vessel counts and collagen I/III ratio were measured, respectively, by light microscopy and Western blot analysis with immunohistochemistry of ghrelin receptors. Estrogen significantly increased urethral and anal vascular counts and collagen I/III ratio in young-adult rats. In old rats, only combined estrogen/ghrelin administration significantly increased both variables. This was not observed with estrogen or ghrelin separately. Ghrelin receptors were immunostained in urethral and anal submucosa of all samples. Combined estrogen/ghrelin administration restored postovariectomy urethral and anal canal submucosal vessel number and collagen I/III ratio in old rats suggesting independent ageing effect.

Original language	English
Pages (from-to)	547-552
Number of pages	6
Journal	International Urogynecology Journal
Volume	19
Issue number	4
DOIs	https://doi.org/10.1007/s00192-007-0462-1
Publication status	Published - Apr 2008

Keywords

Ageing
Estrogen
Ghrelin
Incontinence
Menopause
Rat

ASJC Scopus subject areas

Obstetrics and Gynaecology
Urology

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10.1007/s00192-007-0462-1

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Rizk, D. E. E., Hassan, H. A., Al-Marzouqi, A. H., Ramadan, G. A., Al-Kedrah, S. S., Daoud, S. A., & Fahim, M. A. (2008). Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats. International Urogynecology Journal, 19(4), 547-552. https://doi.org/10.1007/s00192-007-0462-1

Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats. / Rizk, Diaa E.E.; Hassan, Hazem A.; Al-Marzouqi, Ahmed H. et al.
In: International Urogynecology Journal, Vol. 19, No. 4, 04.2008, p. 547-552.

Research output: Contribution to journal › Article › peer-review

Rizk, DEE, Hassan, HA, Al-Marzouqi, AH , Ramadan, GA, Al-Kedrah, SS, Daoud, SA & Fahim, MA 2008, 'Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats', International Urogynecology Journal, vol. 19, no. 4, pp. 547-552. https://doi.org/10.1007/s00192-007-0462-1

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abstract = "We compare the effects of estrogen and/or ghrelin on vascular counts and collagen I/III ratio of urethral and anal canal submucosa in old vs young-adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n=24×2) received 42 daily intraperitoneal 17-β estradiol (10 μg/kg), ghrelin (2 μg/kg), both, or vehicle (n=6×4 per group). Blood vessel counts and collagen I/III ratio were measured, respectively, by light microscopy and Western blot analysis with immunohistochemistry of ghrelin receptors. Estrogen significantly increased urethral and anal vascular counts and collagen I/III ratio in young-adult rats. In old rats, only combined estrogen/ghrelin administration significantly increased both variables. This was not observed with estrogen or ghrelin separately. Ghrelin receptors were immunostained in urethral and anal submucosa of all samples. Combined estrogen/ghrelin administration restored postovariectomy urethral and anal canal submucosal vessel number and collagen I/III ratio in old rats suggesting independent ageing effect.",

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AU - Hassan, Hazem A.

AU - Al-Marzouqi, Ahmed H.

AU - Ramadan, Gaber A.

AU - Al-Kedrah, Soha S.

AU - Daoud, Sayel A.

AU - Fahim, Mohamed A.

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N2 - We compare the effects of estrogen and/or ghrelin on vascular counts and collagen I/III ratio of urethral and anal canal submucosa in old vs young-adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n=24×2) received 42 daily intraperitoneal 17-β estradiol (10 μg/kg), ghrelin (2 μg/kg), both, or vehicle (n=6×4 per group). Blood vessel counts and collagen I/III ratio were measured, respectively, by light microscopy and Western blot analysis with immunohistochemistry of ghrelin receptors. Estrogen significantly increased urethral and anal vascular counts and collagen I/III ratio in young-adult rats. In old rats, only combined estrogen/ghrelin administration significantly increased both variables. This was not observed with estrogen or ghrelin separately. Ghrelin receptors were immunostained in urethral and anal submucosa of all samples. Combined estrogen/ghrelin administration restored postovariectomy urethral and anal canal submucosal vessel number and collagen I/III ratio in old rats suggesting independent ageing effect.

AB - We compare the effects of estrogen and/or ghrelin on vascular counts and collagen I/III ratio of urethral and anal canal submucosa in old vs young-adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n=24×2) received 42 daily intraperitoneal 17-β estradiol (10 μg/kg), ghrelin (2 μg/kg), both, or vehicle (n=6×4 per group). Blood vessel counts and collagen I/III ratio were measured, respectively, by light microscopy and Western blot analysis with immunohistochemistry of ghrelin receptors. Estrogen significantly increased urethral and anal vascular counts and collagen I/III ratio in young-adult rats. In old rats, only combined estrogen/ghrelin administration significantly increased both variables. This was not observed with estrogen or ghrelin separately. Ghrelin receptors were immunostained in urethral and anal submucosa of all samples. Combined estrogen/ghrelin administration restored postovariectomy urethral and anal canal submucosal vessel number and collagen I/III ratio in old rats suggesting independent ageing effect.

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Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats

Abstract

Keywords

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