TY - JOUR
T1 - Comparative mapping in the pig
T2 - Localization of 214 expressed sequence tags
AU - Cirera, Susanna
AU - Jørgensen, Claus B.
AU - Sawera, Milena
AU - Raudsepp, Terje
AU - Chowdhary, Bhanu P.
AU - Fredholm, Merete
PY - 2003/6/1
Y1 - 2003/6/1
N2 - In total, 214 ESTs (Expressed Sequence Tags) were assigned to the porcine gene map by using somatic cell hybrid mapping, radiation hybrid mapping, and FISH. The ESTs were isolated from a porcine small intestine cDNA library on the basis of significant sequence identity with human annotated genes. In total, 390 primer pairs were designed primarily in the 3′ UTR of the sequences. Overall, 58.6% of the ESTs were successfully mapped by this approach. In total, 191 of the localizations are in agreement with the human comparative map, strongly indicating that these represent true orthologous genes. The remaining 23 ESTs provide new comparative mapping data, which should be considered as preliminary until confirmed by other studies. Our mapping efforts provide a significant contribution to the porcine map as well as to the comparative map for human and pig.
AB - In total, 214 ESTs (Expressed Sequence Tags) were assigned to the porcine gene map by using somatic cell hybrid mapping, radiation hybrid mapping, and FISH. The ESTs were isolated from a porcine small intestine cDNA library on the basis of significant sequence identity with human annotated genes. In total, 390 primer pairs were designed primarily in the 3′ UTR of the sequences. Overall, 58.6% of the ESTs were successfully mapped by this approach. In total, 191 of the localizations are in agreement with the human comparative map, strongly indicating that these represent true orthologous genes. The remaining 23 ESTs provide new comparative mapping data, which should be considered as preliminary until confirmed by other studies. Our mapping efforts provide a significant contribution to the porcine map as well as to the comparative map for human and pig.
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U2 - 10.1007/s00335-002-2242-z
DO - 10.1007/s00335-002-2242-z
M3 - Article
C2 - 12879363
AN - SCOPUS:0038358321
SN - 0938-8990
VL - 14
SP - 405
EP - 426
JO - Mammalian Genome
JF - Mammalian Genome
IS - 6
ER -