TY - JOUR
T1 - Comparison of full-length and conserved segments of wheat dehydrin DHN-5 overexpressed in Arabidopsis thaliana showed different responses to abiotic and biotic stress
AU - Drira, Marwa
AU - Hanin, Moez
AU - Masmoudi, Khaled
AU - Brini, Faiçal
PY - 2016
Y1 - 2016
N2 - Dehydrins (DHNs) are among the most common proteins accumulated in plants under water-related stress. They typically contain at least three conserved sequences designated as the Y-, S- and K-segments. The present work aims to highlight the role of the K-segments in plant tolerance to biotic and abiotic stresses. For this purpose, transgenic Arabidopsis thaliana (L.) Heyhn. lines expressing distinct wheat (Triticum aestivum L.) DHN-5 truncated constructs with or without the K-segments were generated. Our results showed that unlike the derivative lacking a K-segment, constructs containing only one or two K-segments enhanced the tolerance of A. thaliana to diverse stresses and were similar to the full-length wheat DHN-5. Moreover, compared with the wild-type and the YS form, the transgenic plants overexpressing wheat DHN-5 with K-segments maintained higher superoxide dismutase, catalase and peroxide dismutase enzymatic activity, and accumulated lower levels of H2O2 and malondialdehyde. In addition, we demonstrated that lines like A. thaliana overexpressing wheat DHN-5 showed increased resistance to fungal infections caused by Botrytis cinerea and Alternaria solani. Finally, the overexpression of the different forms of wheat DHN-5 led to the regulation of the expression of several genes involved in the jasmonic acid signalling pathway.
AB - Dehydrins (DHNs) are among the most common proteins accumulated in plants under water-related stress. They typically contain at least three conserved sequences designated as the Y-, S- and K-segments. The present work aims to highlight the role of the K-segments in plant tolerance to biotic and abiotic stresses. For this purpose, transgenic Arabidopsis thaliana (L.) Heyhn. lines expressing distinct wheat (Triticum aestivum L.) DHN-5 truncated constructs with or without the K-segments were generated. Our results showed that unlike the derivative lacking a K-segment, constructs containing only one or two K-segments enhanced the tolerance of A. thaliana to diverse stresses and were similar to the full-length wheat DHN-5. Moreover, compared with the wild-type and the YS form, the transgenic plants overexpressing wheat DHN-5 with K-segments maintained higher superoxide dismutase, catalase and peroxide dismutase enzymatic activity, and accumulated lower levels of H2O2 and malondialdehyde. In addition, we demonstrated that lines like A. thaliana overexpressing wheat DHN-5 showed increased resistance to fungal infections caused by Botrytis cinerea and Alternaria solani. Finally, the overexpression of the different forms of wheat DHN-5 led to the regulation of the expression of several genes involved in the jasmonic acid signalling pathway.
KW - K-segments
KW - salt stress
KW - transgenic plants
KW - water stress.
UR - http://www.scopus.com/inward/record.url?scp=84992125477&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84992125477&partnerID=8YFLogxK
U2 - 10.1071/FP16134
DO - 10.1071/FP16134
M3 - Article
AN - SCOPUS:84992125477
SN - 1445-4408
VL - 43
SP - 1048
EP - 1060
JO - Functional Plant Biology
JF - Functional Plant Biology
IS - 11
ER -