TY - JOUR
T1 - Comparison of Optimization-Modelling Methods for Metabolites Production in Escherichia coli
AU - Lee, Mee K.
AU - Mohamad, Mohd Saberi
AU - Choon, Yee Wen
AU - Mohd Daud, Kauthar
AU - Nasarudin, Nurul Athirah
AU - Ismail, Mohd Arfian
AU - Ibrahim, Zuwairie
AU - Napis, Suhaimi
AU - Sinnott, Richard O.
PY - 2020/5/6
Y1 - 2020/5/6
N2 - The metabolic network is the reconstruction of the metabolic pathway of an organism that is used to represent the interaction between enzymes and metabolites in genome level. Meanwhile, metabolic engineering is a process that modifies the metabolic network of a cell to increase the production of metabolites. However, the metabolic networks are too complex that cause problem in identifying near-optimal knockout genes/reactions for maximizing the metabolite's production. Therefore, through constraint-based modelling, various metaheuristic algorithms have been improvised to optimize the desired phenotypes. In this paper, PSOMOMA was compared with CSMOMA and ABCMOMA for maximizing the production of succinic acid in E. coli. Furthermore, the results obtained from PSOMOMA were validated with results from the wet lab experiment.
AB - The metabolic network is the reconstruction of the metabolic pathway of an organism that is used to represent the interaction between enzymes and metabolites in genome level. Meanwhile, metabolic engineering is a process that modifies the metabolic network of a cell to increase the production of metabolites. However, the metabolic networks are too complex that cause problem in identifying near-optimal knockout genes/reactions for maximizing the metabolite's production. Therefore, through constraint-based modelling, various metaheuristic algorithms have been improvised to optimize the desired phenotypes. In this paper, PSOMOMA was compared with CSMOMA and ABCMOMA for maximizing the production of succinic acid in E. coli. Furthermore, the results obtained from PSOMOMA were validated with results from the wet lab experiment.
KW - Artificial Intelligence
KW - Bioinformatics
KW - Metabolic Engineering
KW - Metaheuristic algorithms
KW - Minimization of Metabolic Adjustment
UR - http://www.scopus.com/inward/record.url?scp=85086682552&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85086682552&partnerID=8YFLogxK
U2 - 10.1515/jib-2019-0073
DO - 10.1515/jib-2019-0073
M3 - Article
C2 - 32374287
AN - SCOPUS:85086682552
SN - 1613-4516
VL - 17
JO - Journal of integrative bioinformatics
JF - Journal of integrative bioinformatics
IS - 1
ER -