The effects of xenon and halothane on depolarization-induced 45Ca2+ fluxes mediated by voltage-dependent Ca2+ channels were investigated in transverse tubule membrane vesicles from rabbit skeletal muscle. Halothane, in the concentration range of 0.5-2 mM, caused a significant inhibition of 45Ca2+ fluxes. Xenon tested in the range of 60%-100% did not affect the 45Ca2+ fluxes. Radioligand binding studies indicated that xenon and halothane have different effects on the specific binding of [3H]Isradipine to transverse tubule membranes. Halothane caused a significant inhibition on the specific binding of [3H]Isradipine. In controls and in presence of 0.5 mM halothane, Bmax values were 26.9 pmole/mg and 15.1 pmole/mg, and KD values were 238 pM and 247 pM, respectively. On the other hand, there was no effect of xenon (60%-100%) on the characteristics of [3H]Isradipine binding. In conclusion, results indicate that xenon and halothane differ in their effects on the function of voltage-dependent Ca2+ channels and on the specific binding of [3H]Isradipine in skeletal muscle membranes.
|Number of pages||5|
|Journal||Naunyn-Schmiedeberg's Archives of Pharmacology|
|Publication status||Published - 2002|
- Calcium channels
- Skeletal muscle
ASJC Scopus subject areas