Comparison of the effects of xenon and halothane on voltage-dependent Ca2+ fluxes in rabbit T-tubule membranes

Murat Oz, Meral Dinc, Yulia Tchugunova, Susan M. Dunn

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The effects of xenon and halothane on depolarization-induced 45Ca2+ fluxes mediated by voltage-dependent Ca2+ channels were investigated in transverse tubule membrane vesicles from rabbit skeletal muscle. Halothane, in the concentration range of 0.5-2 mM, caused a significant inhibition of 45Ca2+ fluxes. Xenon tested in the range of 60%-100% did not affect the 45Ca2+ fluxes. Radioligand binding studies indicated that xenon and halothane have different effects on the specific binding of [3H]Isradipine to transverse tubule membranes. Halothane caused a significant inhibition on the specific binding of [3H]Isradipine. In controls and in presence of 0.5 mM halothane, Bmax values were 26.9 pmole/mg and 15.1 pmole/mg, and KD values were 238 pM and 247 pM, respectively. On the other hand, there was no effect of xenon (60%-100%) on the characteristics of [3H]Isradipine binding. In conclusion, results indicate that xenon and halothane differ in their effects on the function of voltage-dependent Ca2+ channels and on the specific binding of [3H]Isradipine in skeletal muscle membranes.

Original languageEnglish
Pages (from-to)413-417
Number of pages5
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume365
Issue number5
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Calcium channels
  • Halothane
  • Skeletal muscle
  • Xenon

ASJC Scopus subject areas

  • Pharmacology

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