Composition and properties of flaxseed phenolic oligomers

Christina Strandås; Afaf Kamal-Eldin; Roger Andersson; Per Åman

doi:10.1016/j.foodchem.2008.01.064

Composition and properties of flaxseed phenolic oligomers

Christina Strandås, Afaf Kamal-Eldin, Roger Andersson, Per Åman

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

An extract from flaxseed containing oligomeric structures of the phenolic glucosides secoisolariciresinol diglucoside (SDG), p-coumaric acid glucoside and ferulic acid glucoside was fractionated into three oligomeric fractions (F50, F60 and F70) by reversed phase liquid chromatography and further subfractionated by Sepharose CL-6B. The F50 fraction, which had the highest proportion of hydroxycinnamic acid glucosides, was also fractionated on Sephadex LH-20 according to hydrophobicity and size. The different separations resulted in complex profiles of UV-absorbing molecules. HPLC analyses indicated that reversed-phase chromatography separated the oligomers according to composition of the phenolic glucosides, while the subfractionation revealed that other structural features of the oligomers were also important. Using the DPPH radical, SDG and oligomeric fractions showed similar hydrogen-donating abilities comparable to ferulic acid but higher than α-tocopherol, which suggests that SDG was the only active antioxidant.

Original language	English
Pages (from-to)	106-112
Number of pages	7
Journal	Food Chemistry
Volume	110
Issue number	1
DOIs	https://doi.org/10.1016/j.foodchem.2008.01.064
Publication status	Published - Sept 1 2008
Externally published	Yes

Keywords

Antioxidants
Flaxseed
Lignan
Phenolic glucosides
Phytoestrogen
Secoisolariciresinol diglucoside

ASJC Scopus subject areas

Analytical Chemistry
Food Science

Access to Document

10.1016/j.foodchem.2008.01.064

Cite this

@article{3c1c34839aaa4359bea777c89c7be08f,

title = "Composition and properties of flaxseed phenolic oligomers",

abstract = "An extract from flaxseed containing oligomeric structures of the phenolic glucosides secoisolariciresinol diglucoside (SDG), p-coumaric acid glucoside and ferulic acid glucoside was fractionated into three oligomeric fractions (F50, F60 and F70) by reversed phase liquid chromatography and further subfractionated by Sepharose CL-6B. The F50 fraction, which had the highest proportion of hydroxycinnamic acid glucosides, was also fractionated on Sephadex LH-20 according to hydrophobicity and size. The different separations resulted in complex profiles of UV-absorbing molecules. HPLC analyses indicated that reversed-phase chromatography separated the oligomers according to composition of the phenolic glucosides, while the subfractionation revealed that other structural features of the oligomers were also important. Using the DPPH radical, SDG and oligomeric fractions showed similar hydrogen-donating abilities comparable to ferulic acid but higher than α-tocopherol, which suggests that SDG was the only active antioxidant.",

keywords = "Antioxidants, Flaxseed, Lignan, Phenolic glucosides, Phytoestrogen, Secoisolariciresinol diglucoside",

author = "Christina Strand{\aa}s and Afaf Kamal-Eldin and Roger Andersson and Per {\AA}man",

note = "Funding Information: This project was financed by Formas, the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning.",

year = "2008",

month = sep,

day = "1",

doi = "10.1016/j.foodchem.2008.01.064",

language = "English",

volume = "110",

pages = "106--112",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier Ltd",

number = "1",

}

TY - JOUR

T1 - Composition and properties of flaxseed phenolic oligomers

AU - Strandås, Christina

AU - Kamal-Eldin, Afaf

AU - Andersson, Roger

AU - Åman, Per

N1 - Funding Information: This project was financed by Formas, the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning.

PY - 2008/9/1

Y1 - 2008/9/1

N2 - An extract from flaxseed containing oligomeric structures of the phenolic glucosides secoisolariciresinol diglucoside (SDG), p-coumaric acid glucoside and ferulic acid glucoside was fractionated into three oligomeric fractions (F50, F60 and F70) by reversed phase liquid chromatography and further subfractionated by Sepharose CL-6B. The F50 fraction, which had the highest proportion of hydroxycinnamic acid glucosides, was also fractionated on Sephadex LH-20 according to hydrophobicity and size. The different separations resulted in complex profiles of UV-absorbing molecules. HPLC analyses indicated that reversed-phase chromatography separated the oligomers according to composition of the phenolic glucosides, while the subfractionation revealed that other structural features of the oligomers were also important. Using the DPPH radical, SDG and oligomeric fractions showed similar hydrogen-donating abilities comparable to ferulic acid but higher than α-tocopherol, which suggests that SDG was the only active antioxidant.

AB - An extract from flaxseed containing oligomeric structures of the phenolic glucosides secoisolariciresinol diglucoside (SDG), p-coumaric acid glucoside and ferulic acid glucoside was fractionated into three oligomeric fractions (F50, F60 and F70) by reversed phase liquid chromatography and further subfractionated by Sepharose CL-6B. The F50 fraction, which had the highest proportion of hydroxycinnamic acid glucosides, was also fractionated on Sephadex LH-20 according to hydrophobicity and size. The different separations resulted in complex profiles of UV-absorbing molecules. HPLC analyses indicated that reversed-phase chromatography separated the oligomers according to composition of the phenolic glucosides, while the subfractionation revealed that other structural features of the oligomers were also important. Using the DPPH radical, SDG and oligomeric fractions showed similar hydrogen-donating abilities comparable to ferulic acid but higher than α-tocopherol, which suggests that SDG was the only active antioxidant.

KW - Antioxidants

KW - Flaxseed

KW - Lignan

KW - Phenolic glucosides

KW - Phytoestrogen

KW - Secoisolariciresinol diglucoside

UR - http://www.scopus.com/inward/record.url?scp=41549135044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41549135044&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2008.01.064

DO - 10.1016/j.foodchem.2008.01.064

M3 - Article

AN - SCOPUS:41549135044

SN - 0308-8146

VL - 110

SP - 106

EP - 112

JO - Food Chemistry

JF - Food Chemistry

IS - 1

ER -

Composition and properties of flaxseed phenolic oligomers

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this