TY - JOUR
T1 - Conductometric immunosensor for escherichia coli o157:H7 detection based on polyaniline/zinc oxide (pani/zno) nanocomposite
AU - Mutlaq, Sawsan
AU - Albiss, Borhan
AU - Al-Nabulsi, Anas A.
AU - Jaradat, Ziad W.
AU - Olaimat, Amin N.
AU - Khalifeh, Mohammad S.
AU - Osaili, Tareq
AU - Ayyash, Mutamed M.
AU - Holley, Richard A.
N1 - Funding Information:
Funding: This research was funded by the deanship of research at Jordan University of Science and Technology, Jordan, grant number 2020-113.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/10/1
Y1 - 2021/10/1
N2 - A conductometric immunosensor was developed for the detection of one of the most common foodborne pathogens, Escherichia coli O157:H7 (E. coli O157:H7), by conductometric sensing. The sensor was built based on a polyaniline/zinc oxide (PANI/ZnO) nanocomposite film spin-coated on a gold electrode. Then, it was modified with a monoclonal anti-E. coli O157:H7 antibody as a biorecognition element. The fabricated nanostructured sensor was able to quantify the pathogens under optimal detection conditions, within 30 min, and showed a good detection range from 101 to 104 CFU/mL for E. coli O157:H7 and a minimum detection limit of 4.8 CFU/mL in 0.1% peptone water. The sensor efficiency for detecting bacteria in food matrices was tested in ultra-heat-treated (UHT) skim milk. E. coli O157:H7 was detected at concentrations of 101 to 104 CFU/mL with a minimum detection limit of 13.9 CFU/mL. The novel sensor was simple, fast, highly sensitive with excellent specificity, and it had the potential for rapid sample processing. Moreover, this unique technique for bacterial detection could be applicable for food safety and quality control in the food sector as it offers highly reliable results and is able to quantify the target bacterium.
AB - A conductometric immunosensor was developed for the detection of one of the most common foodborne pathogens, Escherichia coli O157:H7 (E. coli O157:H7), by conductometric sensing. The sensor was built based on a polyaniline/zinc oxide (PANI/ZnO) nanocomposite film spin-coated on a gold electrode. Then, it was modified with a monoclonal anti-E. coli O157:H7 antibody as a biorecognition element. The fabricated nanostructured sensor was able to quantify the pathogens under optimal detection conditions, within 30 min, and showed a good detection range from 101 to 104 CFU/mL for E. coli O157:H7 and a minimum detection limit of 4.8 CFU/mL in 0.1% peptone water. The sensor efficiency for detecting bacteria in food matrices was tested in ultra-heat-treated (UHT) skim milk. E. coli O157:H7 was detected at concentrations of 101 to 104 CFU/mL with a minimum detection limit of 13.9 CFU/mL. The novel sensor was simple, fast, highly sensitive with excellent specificity, and it had the potential for rapid sample processing. Moreover, this unique technique for bacterial detection could be applicable for food safety and quality control in the food sector as it offers highly reliable results and is able to quantify the target bacterium.
KW - Antibody
KW - E. coli O157:H7
KW - Immunosensor
KW - Polyaniline
KW - Zinc oxide nanoparticles
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U2 - 10.3390/polym13193288
DO - 10.3390/polym13193288
M3 - Article
AN - SCOPUS:85116009721
SN - 2073-4360
VL - 13
JO - Polymers
JF - Polymers
IS - 19
M1 - 3288
ER -