TY - JOUR
T1 - Conserved spinal cord bioenergetics in experimental autoimmune encephalomyelitis in C57BL6 mice, measured using phosphorescence oxygen analyzer
AU - Al Shamsi, Mariam
AU - Shahin, Allen
AU - Kamyan, Doua
AU - Alnaqbi, Alanood
AU - Shaban, Sami
AU - Souid, Abdul Kader
N1 - Funding Information:
This work was supported by a grant from the College of Medicine & Health Sciences, UAE University.
Publisher Copyright:
© 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
PY - 2021/10
Y1 - 2021/10
N2 - Background: We have previously reported that spinal cord respiration (cellular mitochondrial oxygen consump-tion) and ATP content are conserved in the studied model of experimental autoimmune encephalomyelitis (EAE), foreseeing a recovery of the diseased rats. This exemplary lesion of multiple sclerosis is used here to measure spinal cord bioenergetics in C57BL6 mice. Our hypothesis is that, despite the well-known focal axonal mito-chondrial pathology, bioenergetics of the CNS is reasonably preserved in this disease. Methods: EAE was induced with an immunodominant myelin oligodendrocyte glycoprotein epitope in complete Freund's adjuvant, appended by injections of pertussis toxin. A low and high-dose of the encephalitogen, administered into base of tail or hind-flank, were investigated. Control mice received only the incomplete adjuvant into tail. Oxygen measurements were based on quenching the phosphorescence of Pd(II) meso-tetra (sulfophenyl) tetrabenzoporphyrin by molecular oxygen. Cellular ATP was measured using the luciferin/lucif-erase system. Results: The kinetics of spinal cord oxygen consumption was zero-order (linear with time) and inhibited by cy-anide, confirming oxygen was reduced by cytochrome oxidase. The rate of respiration (in μMO2.min-1.mg-1; measured on Days 13–28) in control mice was (mean ± SD) 0.086 ± 0.024 (n = 8) and in immunized mice was 0.079 ± 0.020 (n = 15, P = 0.265, Mann-Whitney test). Consistently, cellular ATP (in μmol mg-1 dry pellet weight; measured on Days 13–28) in control mice was 0.068 ± 0.079 (n = 11) and in immunized mice was 0.063 ± 0.061 (n = 24, P = 0.887, Mann-Whitney U test). Conclusions: In vitro measurements of spinal cord bioenergetics show conservation of the mitochondrial function in mice with EAE. These results suggest the previously documented reduced mitochondrial electrochemical potential in this disease is alterable, and likely reflects the adverse events of neuroinflammation.
AB - Background: We have previously reported that spinal cord respiration (cellular mitochondrial oxygen consump-tion) and ATP content are conserved in the studied model of experimental autoimmune encephalomyelitis (EAE), foreseeing a recovery of the diseased rats. This exemplary lesion of multiple sclerosis is used here to measure spinal cord bioenergetics in C57BL6 mice. Our hypothesis is that, despite the well-known focal axonal mito-chondrial pathology, bioenergetics of the CNS is reasonably preserved in this disease. Methods: EAE was induced with an immunodominant myelin oligodendrocyte glycoprotein epitope in complete Freund's adjuvant, appended by injections of pertussis toxin. A low and high-dose of the encephalitogen, administered into base of tail or hind-flank, were investigated. Control mice received only the incomplete adjuvant into tail. Oxygen measurements were based on quenching the phosphorescence of Pd(II) meso-tetra (sulfophenyl) tetrabenzoporphyrin by molecular oxygen. Cellular ATP was measured using the luciferin/lucif-erase system. Results: The kinetics of spinal cord oxygen consumption was zero-order (linear with time) and inhibited by cy-anide, confirming oxygen was reduced by cytochrome oxidase. The rate of respiration (in μMO2.min-1.mg-1; measured on Days 13–28) in control mice was (mean ± SD) 0.086 ± 0.024 (n = 8) and in immunized mice was 0.079 ± 0.020 (n = 15, P = 0.265, Mann-Whitney test). Consistently, cellular ATP (in μmol mg-1 dry pellet weight; measured on Days 13–28) in control mice was 0.068 ± 0.079 (n = 11) and in immunized mice was 0.063 ± 0.061 (n = 24, P = 0.887, Mann-Whitney U test). Conclusions: In vitro measurements of spinal cord bioenergetics show conservation of the mitochondrial function in mice with EAE. These results suggest the previously documented reduced mitochondrial electrochemical potential in this disease is alterable, and likely reflects the adverse events of neuroinflammation.
KW - Cellular bioenergetics
KW - Cellular respiration
KW - Energy conversion
KW - Multiple sclerosis
KW - Neurons
KW - Oxygen measurements
KW - Spinal cord
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U2 - 10.1016/j.heliyon.2021.e08111
DO - 10.1016/j.heliyon.2021.e08111
M3 - Article
AN - SCOPUS:85120859875
SN - 2405-8440
VL - 7
JO - Heliyon
JF - Heliyon
IS - 10
M1 - e08111
ER -