Construction of a medium-density horse gene map

M. Perrocheau; V. Boutreux; S. Chadi; X. Mata; P. Decaunes; T. Raudsepp; K. Durkin; D. Incarnato; L. Iannuzzi; T. L. Lear; K. Hirota; T. Hasegawa; B. Zhu; P. De Jong; E. P. Cribiu; B. P. Chowdhary; G. Guérin

doi:10.1111/j.1365-2052.2005.01401.x

Construction of a medium-density horse gene map

M. Perrocheau, V. Boutreux, S. Chadi, X. Mata, P. Decaunes, T. Raudsepp, K. Durkin, D. Incarnato, L. Iannuzzi, T. L. Lear, K. Hirota, T. Hasegawa, B. Zhu, P. De Jong, E. P. Cribiu, B. P. Chowdhary, G. Guérin

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the INRA and the CHORI-241 equine BAC libraries by polymerase chain reaction and by filter hybridization respectively. Two hundred and thirty-seven BACs containing equine gene orthologues, confirmed by sequencing, were isolated. The BACs were localized to horse chromosomes by fluorescent in situ hybridization (FISH). Overall, 165 genes were assigned to the equine genomic map by radiation hybrid (RH) (using an equine RH₅₀₀₀ panel) and/or by FISH mapping. A comparison of localizations of 713 genes mapped on the horse genome and on the human genome revealed 59 homologous segments and 131 conserved segments. Two of these homologies (ECA27/HSA8 and ECA12p/HSA11p) had not been previously identified. An enhanced resolution of conserved and rearranged chromosomal segments presented in this study provides clarification of chromosome evolution history.

Original language	English
Pages (from-to)	145-155
Number of pages	11
Journal	Animal Genetics
Volume	37
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2052.2005.01401.x
Publication status	Published - Apr 2006
Externally published	Yes

Keywords

Comparative mapping
Gene mapping
Horse

ASJC Scopus subject areas

Animal Science and Zoology
Genetics

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10.1111/j.1365-2052.2005.01401.x

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title = "Construction of a medium-density horse gene map",

abstract = "A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the INRA and the CHORI-241 equine BAC libraries by polymerase chain reaction and by filter hybridization respectively. Two hundred and thirty-seven BACs containing equine gene orthologues, confirmed by sequencing, were isolated. The BACs were localized to horse chromosomes by fluorescent in situ hybridization (FISH). Overall, 165 genes were assigned to the equine genomic map by radiation hybrid (RH) (using an equine RH5000 panel) and/or by FISH mapping. A comparison of localizations of 713 genes mapped on the horse genome and on the human genome revealed 59 homologous segments and 131 conserved segments. Two of these homologies (ECA27/HSA8 and ECA12p/HSA11p) had not been previously identified. An enhanced resolution of conserved and rearranged chromosomal segments presented in this study provides clarification of chromosome evolution history.",

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author = "M. Perrocheau and V. Boutreux and S. Chadi and X. Mata and P. Decaunes and T. Raudsepp and K. Durkin and D. Incarnato and L. Iannuzzi and Lear, {T. L.} and K. Hirota and T. Hasegawa and B. Zhu and {De Jong}, P. and Cribiu, {E. P.} and Chowdhary, {B. P.} and G. Gu{\'e}rin",

year = "2006",

month = apr,

doi = "10.1111/j.1365-2052.2005.01401.x",

language = "English",

volume = "37",

pages = "145--155",

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T1 - Construction of a medium-density horse gene map

AU - Perrocheau, M.

AU - Boutreux, V.

AU - Chadi, S.

AU - Mata, X.

AU - Decaunes, P.

AU - Raudsepp, T.

AU - Durkin, K.

AU - Incarnato, D.

AU - Iannuzzi, L.

AU - Lear, T. L.

AU - Hirota, K.

AU - Hasegawa, T.

AU - Zhu, B.

AU - De Jong, P.

AU - Cribiu, E. P.

AU - Chowdhary, B. P.

AU - Guérin, G.

PY - 2006/4

Y1 - 2006/4

N2 - A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the INRA and the CHORI-241 equine BAC libraries by polymerase chain reaction and by filter hybridization respectively. Two hundred and thirty-seven BACs containing equine gene orthologues, confirmed by sequencing, were isolated. The BACs were localized to horse chromosomes by fluorescent in situ hybridization (FISH). Overall, 165 genes were assigned to the equine genomic map by radiation hybrid (RH) (using an equine RH5000 panel) and/or by FISH mapping. A comparison of localizations of 713 genes mapped on the horse genome and on the human genome revealed 59 homologous segments and 131 conserved segments. Two of these homologies (ECA27/HSA8 and ECA12p/HSA11p) had not been previously identified. An enhanced resolution of conserved and rearranged chromosomal segments presented in this study provides clarification of chromosome evolution history.

AB - A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the INRA and the CHORI-241 equine BAC libraries by polymerase chain reaction and by filter hybridization respectively. Two hundred and thirty-seven BACs containing equine gene orthologues, confirmed by sequencing, were isolated. The BACs were localized to horse chromosomes by fluorescent in situ hybridization (FISH). Overall, 165 genes were assigned to the equine genomic map by radiation hybrid (RH) (using an equine RH5000 panel) and/or by FISH mapping. A comparison of localizations of 713 genes mapped on the horse genome and on the human genome revealed 59 homologous segments and 131 conserved segments. Two of these homologies (ECA27/HSA8 and ECA12p/HSA11p) had not been previously identified. An enhanced resolution of conserved and rearranged chromosomal segments presented in this study provides clarification of chromosome evolution history.

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KW - Gene mapping

KW - Horse

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Construction of a medium-density horse gene map

Abstract

Keywords

ASJC Scopus subject areas

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