CRISPR-Cas12-based nucleic acids detection systems

Ross Ka Kit Leung, Qiu Xiang Cheng, Zhi Le Wu, Gulfaraz Khan, Yang Liu, Hai Yang Xia, Jin Wang

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Because of the outstanding contribution in genome editing, CRISPR has undoubtedly become the most popular technology around the world and two pioneers are awarded the Nobel Prize in Chemistry this year. Besides, along with the discovery of nonspecific trans-cleavage activities of several Cas proteins such as Cas12 and Cas13, many CRISPR-based molecular diagnostic systems have been successfully created, showing advantages in sensitivity, specificity and operation convenience. Among them, systems with Cas12, which targets DNA and trans-cleaves single-stranded DNA probes, are both simple and highly efficient. Here in this review, we mainly focus on the Cas12-based methods and briefly discuss their applications in nucleic acids detection and beyond.

Original languageEnglish
Pages (from-to)276-281
Number of pages6
Publication statusPublished - Jul 2022


  • CRISPR diagnostics
  • Cas12
  • Trans-cleavage

ASJC Scopus subject areas

  • Molecular Biology
  • General Biochemistry,Genetics and Molecular Biology


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