@article{a512df1c5c754440802721deba9da25f,
title = "CRISPR-Cas12-based nucleic acids detection systems",
abstract = "Because of the outstanding contribution in genome editing, CRISPR has undoubtedly become the most popular technology around the world and two pioneers are awarded the Nobel Prize in Chemistry this year. Besides, along with the discovery of nonspecific trans-cleavage activities of several Cas proteins such as Cas12 and Cas13, many CRISPR-based molecular diagnostic systems have been successfully created, showing advantages in sensitivity, specificity and operation convenience. Among them, systems with Cas12, which targets DNA and trans-cleaves single-stranded DNA probes, are both simple and highly efficient. Here in this review, we mainly focus on the Cas12-based methods and briefly discuss their applications in nucleic acids detection and beyond.",
keywords = "CRISPR, CRISPR diagnostics, Cas12, HOLMES, Trans-cleavage",
author = "Leung, {Ross Ka Kit} and Cheng, {Qiu Xiang} and Wu, {Zhi Le} and Gulfaraz Khan and Yang Liu and Xia, {Hai Yang} and Jin Wang",
note = "Funding Information: We thank the members of Wang lab of the Institute of Translational Medicine, Shenzhen Second People{\textquoteright}s Hospital for many fruitful and inspiring discussions. This work was supported by grants from the National Natural Science Foundation of China ( 31922046 ), Sanming Project of Medicine in Shenzhen ( SZSM202011017 ), the Commissioned Health and Medical Research Fund of Food and Health Bureau of HKSAR Government ( COVID190102 ), Special Program for Technical Support of the State Administration for Market Regulation (2019YJ018), UAEU (31R0135 and 31R246), Al Jalila Foundation ( 21M132 ) and the Scientific Research Foundation of Taizhou (No. 2001xg07 ). The sponsors have no involvement in the study design; collection, analysis and interpretation of data; the writing of the manuscript; the decision to submit the manuscript for publication. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2022",
month = jul,
doi = "10.1016/j.ymeth.2021.02.018",
language = "English",
volume = "203",
pages = "276--281",
journal = "Methods",
issn = "1046-2023",
publisher = "Academic Press Inc.",
}