CRISPR workflow solutions: Cargos and versatile delivery platforms in genome editing

Ahmad Munir, Muhammad Ali, Sameer H. Qari, Nayla Munawar, Muhammad Sulyman Saleem, Aftab Ahmad

Research output: Chapter in Book/Report/Conference proceedingChapter


Genome editing through CRISPR-Cas has revolutionized the landscape of biological sciences by enabling scientists to engineer plant and animal genomes in a precise manner for desired traits. CRISPR has been demonstrated with successful proof of concept studies in plants for disease resistance, stress tolerance, gene regulation, base editing, chromatin imaging and epigenome editing. Although several CRISPR crops have been approved for commercialization and CRISPR holds great potential for food security and genetic improvement of crops, applications of CRISPR-Cas are limited efficient delivery of CRISPR reagents in plant cells. CRISPR reagents have been delivered in the form of DNA, mRNA and RNPs, delivered through various delivery methods. In this chapter, we summarize different CRISPR reagents along with their merits and demerits in downstream applications. In addition, we discuss different delivery methods such as viral, nonviral and physical methods, used for CRISPR cargos in genome editing. Moreover, we highlight, a comparison of different delivery systems for efficient genome editing. Furthermore, we also summarize emerging delivery methods for CRISPR-mediated genome editing. Finally, we discuss the challenges and prospects of efficient delivery of CRISPR cargoes to achieve genome editing.

Original languageEnglish
Title of host publicationCRISPRized Horticulture Crops
Subtitle of host publicationGenome Modified Plants and Microbes in Food and Agriculture
Number of pages24
ISBN (Electronic)9780443132292
ISBN (Print)9780443132308
Publication statusPublished - Jan 1 2024


  • CRISPR-Cas9
  • Delivery systems
  • Gene editing
  • Nonviral vectors

ASJC Scopus subject areas

  • General Engineering
  • General Agricultural and Biological Sciences


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