Cyclic AMP-mediated release of peptide YY (PYY) from the isolated perfused rabbit distal colon

Garth H. Ballantyne; James R. Goldenring; Paul E. Savoca; Heidi K. Kranz; Thomas E. Adrian; Anton J. Bilchik; Irvin M. Modlin

doi:10.1016/0167-0115(93)90415-5

Cyclic AMP-mediated release of peptide YY (PYY) from the isolated perfused rabbit distal colon

Garth H. Ballantyne, James R. Goldenring, Paul E. Savoca, Heidi K. Kranz, Thomas E. Adrian, Anton J. Bilchik, Irvin M. Modlin

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We have investigated the role of the colonic nervous system in regulating the release of peptide YY (PYY) from the isolated perfused rabbit distal colon. In addition, we have studied the role of cAMP- and Ca²⁺-dependent mechanisms in mediating the release of PYY. We investigated three agents which stimulate increases in intracellular cAMP (vasoactive intestinal polypeptide (VIP), cholera toxin, and forskolin) and three agents which raise intracellular Ca²⁺ concentrations (substance P, carbachol, and the calcium ionophore A23187). The three cAMP-dependent agents, VIP (10^-7 M and 3·10^-7 M), cholera toxin (100 μg), and forskolin (10^-6 M and 10^-5 M) significantly stimulated release of PYY (P<0.05). Tetrodotoxin (3·10^-6 M) did not alter forskolin (10^-5 M) stimulated release of PYY. Substance P (10^-9 M and 10^-8 M), carbachol (10^-5 M), and A23187 (10^-6 M) failed to stimulate the release of PYY. These results suggest that the colonic neurotransmitter VIP participates in the modulation of PYY release from rabbit distal colons. Further, these studies suggest that release of PYY is mediated by cAMP-dependent pathways.

Original language	English
Pages (from-to)	117-126
Number of pages	10
Journal	Regulatory Peptides
Volume	47
Issue number	2
DOIs	https://doi.org/10.1016/0167-0115(93)90415-5
Publication status	Published - Sept 3 1993
Externally published	Yes

Keywords

A23187
Carbachol
Cholera toxin
Gut hormone
Neuropeptide
Rabbit
Substance P
Tetrodotoxin
Vasoactive intestinal peptide

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/0167-0115(93)90415-5

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@article{7354bff089884bfb8ccd3a6d90a63bc1,

title = "Cyclic AMP-mediated release of peptide YY (PYY) from the isolated perfused rabbit distal colon",

abstract = "We have investigated the role of the colonic nervous system in regulating the release of peptide YY (PYY) from the isolated perfused rabbit distal colon. In addition, we have studied the role of cAMP- and Ca2+-dependent mechanisms in mediating the release of PYY. We investigated three agents which stimulate increases in intracellular cAMP (vasoactive intestinal polypeptide (VIP), cholera toxin, and forskolin) and three agents which raise intracellular Ca2+ concentrations (substance P, carbachol, and the calcium ionophore A23187). The three cAMP-dependent agents, VIP (10-7 M and 3·10-7 M), cholera toxin (100 μg), and forskolin (10-6 M and 10-5 M) significantly stimulated release of PYY (P<0.05). Tetrodotoxin (3·10-6 M) did not alter forskolin (10-5 M) stimulated release of PYY. Substance P (10-9 M and 10-8 M), carbachol (10-5 M), and A23187 (10-6 M) failed to stimulate the release of PYY. These results suggest that the colonic neurotransmitter VIP participates in the modulation of PYY release from rabbit distal colons. Further, these studies suggest that release of PYY is mediated by cAMP-dependent pathways.",

keywords = "A23187, Carbachol, Cholera toxin, Gut hormone, Neuropeptide, Rabbit, Substance P, Tetrodotoxin, Vasoactive intestinal peptide",

author = "Ballantyne, {Garth H.} and Goldenring, {James R.} and Savoca, {Paul E.} and Kranz, {Heidi K.} and Adrian, {Thomas E.} and Bilchik, {Anton J.} and Modlin, {Irvin M.}",

note = "Funding Information: We greatly appreciated the technical assistance provided by Mark Barreuther in performing these studies. This study was supported by a Veterans Administration Merit Award to GHB and the support of the Charles Ohse Research Fund and Saint Mary's Hospital, Waterbury, CT.",

year = "1993",

month = sep,

day = "3",

doi = "10.1016/0167-0115(93)90415-5",

language = "English",

volume = "47",

pages = "117--126",

journal = "Regulatory Peptides",

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T1 - Cyclic AMP-mediated release of peptide YY (PYY) from the isolated perfused rabbit distal colon

AU - Ballantyne, Garth H.

AU - Goldenring, James R.

AU - Savoca, Paul E.

AU - Kranz, Heidi K.

AU - Adrian, Thomas E.

AU - Bilchik, Anton J.

AU - Modlin, Irvin M.

N1 - Funding Information: We greatly appreciated the technical assistance provided by Mark Barreuther in performing these studies. This study was supported by a Veterans Administration Merit Award to GHB and the support of the Charles Ohse Research Fund and Saint Mary's Hospital, Waterbury, CT.

PY - 1993/9/3

Y1 - 1993/9/3

N2 - We have investigated the role of the colonic nervous system in regulating the release of peptide YY (PYY) from the isolated perfused rabbit distal colon. In addition, we have studied the role of cAMP- and Ca2+-dependent mechanisms in mediating the release of PYY. We investigated three agents which stimulate increases in intracellular cAMP (vasoactive intestinal polypeptide (VIP), cholera toxin, and forskolin) and three agents which raise intracellular Ca2+ concentrations (substance P, carbachol, and the calcium ionophore A23187). The three cAMP-dependent agents, VIP (10-7 M and 3·10-7 M), cholera toxin (100 μg), and forskolin (10-6 M and 10-5 M) significantly stimulated release of PYY (P<0.05). Tetrodotoxin (3·10-6 M) did not alter forskolin (10-5 M) stimulated release of PYY. Substance P (10-9 M and 10-8 M), carbachol (10-5 M), and A23187 (10-6 M) failed to stimulate the release of PYY. These results suggest that the colonic neurotransmitter VIP participates in the modulation of PYY release from rabbit distal colons. Further, these studies suggest that release of PYY is mediated by cAMP-dependent pathways.

AB - We have investigated the role of the colonic nervous system in regulating the release of peptide YY (PYY) from the isolated perfused rabbit distal colon. In addition, we have studied the role of cAMP- and Ca2+-dependent mechanisms in mediating the release of PYY. We investigated three agents which stimulate increases in intracellular cAMP (vasoactive intestinal polypeptide (VIP), cholera toxin, and forskolin) and three agents which raise intracellular Ca2+ concentrations (substance P, carbachol, and the calcium ionophore A23187). The three cAMP-dependent agents, VIP (10-7 M and 3·10-7 M), cholera toxin (100 μg), and forskolin (10-6 M and 10-5 M) significantly stimulated release of PYY (P<0.05). Tetrodotoxin (3·10-6 M) did not alter forskolin (10-5 M) stimulated release of PYY. Substance P (10-9 M and 10-8 M), carbachol (10-5 M), and A23187 (10-6 M) failed to stimulate the release of PYY. These results suggest that the colonic neurotransmitter VIP participates in the modulation of PYY release from rabbit distal colons. Further, these studies suggest that release of PYY is mediated by cAMP-dependent pathways.

KW - A23187

KW - Carbachol

KW - Cholera toxin

KW - Gut hormone

KW - Neuropeptide

KW - Rabbit

KW - Substance P

KW - Tetrodotoxin

KW - Vasoactive intestinal peptide

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SN - 0167-0115

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JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 2

ER -

Cyclic AMP-mediated release of peptide YY (PYY) from the isolated perfused rabbit distal colon

Abstract

Keywords

ASJC Scopus subject areas

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