TY - JOUR
T1 - Cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by Bifidobacterium species
T2 - In-vitro investigations
AU - Ayyash, Mutamed
AU - Johnson, Stuart K.
AU - Liu, Shao Quan
AU - Al-Mheiri, Aysha
AU - Abushelaibi, Aisha
N1 - Funding Information:
Authors would like to thank UAE University (Grant No. G00002238 ) for funding this project. Authors are also thankful to Dr Jaleel Kizhakkayil and Ms Suhier Al-Mahadin for technical support.
Funding Information:
Authors would like to thank UAE University (Grant No. G00002238) for funding this project. Authors are also thankful to Dr Jaleel Kizhakkayil and Ms Suhier Al-Mahadin for technical support.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/9
Y1 - 2018/9
N2 - This study aimed to investigate the in-vitro cytotoxicity activity against two cancer cell lines, α-amylase and α-glucosidase inhibitions, ACE-inhibition, antioxidant and proteolytic activities of whole-grain lupin, quinoa and wheat fermented by three species of Bifidobacterium spp. B. animalis, B. breve and B. longum were employed in solid-state fermentation (SSF) 37 °C for 72 h. The ranges of total phenolic contents in fermented lupin (FL), quinoa (FQ) and wheat (FW) were ∼17.0 to 44.0, ∼11.0 to 36.0, ∼5.0–29.0 mg GAE/g, respectively, relative to the unfermented control. α-Glucosidase inhibition increased significantly (p < 0.05) in FL and FQ after 24 h of fermentation. The cereals fermented by B. breve and B. longum had markedly increased ACE-inhibition activities. The magnitude of the cytotoxicity activities in FL was greater (p < 0.05) by approximately 5-fold and 3-fold against colon cancer cell (Caco-2) and breast cancer cell (MCF-7), respectively than in FQ and FW. The findings suggest that health properties of grains could be enhanced by probiotic fermentation and such enhancements depend on grain type and probiotic species. The inclusion of grains fermented by probiotics into foods could improve health benefits of these product.
AB - This study aimed to investigate the in-vitro cytotoxicity activity against two cancer cell lines, α-amylase and α-glucosidase inhibitions, ACE-inhibition, antioxidant and proteolytic activities of whole-grain lupin, quinoa and wheat fermented by three species of Bifidobacterium spp. B. animalis, B. breve and B. longum were employed in solid-state fermentation (SSF) 37 °C for 72 h. The ranges of total phenolic contents in fermented lupin (FL), quinoa (FQ) and wheat (FW) were ∼17.0 to 44.0, ∼11.0 to 36.0, ∼5.0–29.0 mg GAE/g, respectively, relative to the unfermented control. α-Glucosidase inhibition increased significantly (p < 0.05) in FL and FQ after 24 h of fermentation. The cereals fermented by B. breve and B. longum had markedly increased ACE-inhibition activities. The magnitude of the cytotoxicity activities in FL was greater (p < 0.05) by approximately 5-fold and 3-fold against colon cancer cell (Caco-2) and breast cancer cell (MCF-7), respectively than in FQ and FW. The findings suggest that health properties of grains could be enhanced by probiotic fermentation and such enhancements depend on grain type and probiotic species. The inclusion of grains fermented by probiotics into foods could improve health benefits of these product.
KW - Antihypertensive
KW - Cytotoxicity
KW - Fermentation
KW - Grain
KW - Probiotic
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U2 - 10.1016/j.lwt.2018.04.099
DO - 10.1016/j.lwt.2018.04.099
M3 - Article
AN - SCOPUS:85046677324
SN - 0023-6438
VL - 95
SP - 295
EP - 302
JO - LWT
JF - LWT
ER -