Dental pulp stem cells ameliorate D-galactose-induced cardiac ageing in rats

Gehan El-Akabawy, Sherif Othman Fathy El-Kersh, Ahmed Othman Fathy Othman El-Kersh, Shaimaa Nasr Amin, Laila Ahmed Rashed, Noha Abdel Latif, Ahmed Elshamey, Mohamed Abdallah Abd El Megied Abdallah, Ibrahim G. Saleh, Zaw Myo Hein, Ibrahim El-Serafi, Nabil Eid

Research output: Contribution to journalArticlepeer-review


Background. Ageing is a key risk factor for cardiovascular disease and is linked to several alterations in cardiac structure and function, including left ventricular hypertrophy and increased cardiomyocyte volume, as well as a decline in the number of cardiomyocytes and ventricular dysfunction, emphasizing the pathological impacts of cardiomyocyte ageing. Dental pulp stem cells (DPSCs) are promising as a cellular therapeutic source due to their minimally invasive surgical approach and remarkable proliferative ability. Aim. This study is the first to investigate the outcomes of the systemic transplantation of DPSCs in a D-galactose (D-gal)-induced rat model of cardiac ageing. Methods. Thirty 9-week-old Sprague-Dawley male rats were randomly assigned into three groups: control, ageing (D-gal), and transplanted groups (D-gal + DPSCs). D-gal (300 mg/kg/day) was administered intraperitoneally daily for 8 weeks. The rats in the transplantation group were intravenously injected with DPSCs at a dose of 1 × 106 once every 2 weeks. Results. The transplanted cells migrated to the heart, differentiated into cardiomyocytes, improved cardiac function, upregulated Sirt1 expression, exerted antioxidative effects, modulated connexin-43 expression, attenuated cardiac histopathological alterations, and had anti-senescent and anti-apoptotic effects. Conclusion. Our results reveal the beneficial effects of DPSC transplantation in a cardiac ageing rat model, suggesting their potential as a viable cell therapy for ageing hearts.

Original languageEnglish
Article numbere17299
Issue number5
Publication statusPublished - 2024


  • Cardiac aging
  • D-galactose
  • Dental pulp stem cell
  • Rat model

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


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