TY - JOUR
T1 - Dental pulp stem cells ameliorate D-galactose-induced cardiac ageing in rats
AU - El-Akabawy, Gehan
AU - El-Kersh, Sherif Othman Fathy
AU - El-Kersh, Ahmed Othman Fathy Othman
AU - Amin, Shaimaa Nasr
AU - Rashed, Laila Ahmed
AU - Latif, Noha Abdel
AU - Elshamey, Ahmed
AU - El Megied Abdallah, Mohamed Abdallah Abd
AU - Saleh, Ibrahim G.
AU - Hein, Zaw Myo
AU - El-Serafi, Ibrahim
AU - Eid, Nabil
N1 - Publisher Copyright:
Copyright 2024 El-Akabawy et al.
PY - 2024
Y1 - 2024
N2 - Background. Ageing is a key risk factor for cardiovascular disease and is linked to several alterations in cardiac structure and function, including left ventricular hypertrophy and increased cardiomyocyte volume, as well as a decline in the number of cardiomyocytes and ventricular dysfunction, emphasizing the pathological impacts of cardiomyocyte ageing. Dental pulp stem cells (DPSCs) are promising as a cellular therapeutic source due to their minimally invasive surgical approach and remarkable proliferative ability. Aim. This study is the first to investigate the outcomes of the systemic transplantation of DPSCs in a D-galactose (D-gal)-induced rat model of cardiac ageing. Methods. Thirty 9-week-old Sprague-Dawley male rats were randomly assigned into three groups: control, ageing (D-gal), and transplanted groups (D-gal + DPSCs). D-gal (300 mg/kg/day) was administered intraperitoneally daily for 8 weeks. The rats in the transplantation group were intravenously injected with DPSCs at a dose of 1 × 106 once every 2 weeks. Results. The transplanted cells migrated to the heart, differentiated into cardiomyocytes, improved cardiac function, upregulated Sirt1 expression, exerted antioxidative effects, modulated connexin-43 expression, attenuated cardiac histopathological alterations, and had anti-senescent and anti-apoptotic effects. Conclusion. Our results reveal the beneficial effects of DPSC transplantation in a cardiac ageing rat model, suggesting their potential as a viable cell therapy for ageing hearts.
AB - Background. Ageing is a key risk factor for cardiovascular disease and is linked to several alterations in cardiac structure and function, including left ventricular hypertrophy and increased cardiomyocyte volume, as well as a decline in the number of cardiomyocytes and ventricular dysfunction, emphasizing the pathological impacts of cardiomyocyte ageing. Dental pulp stem cells (DPSCs) are promising as a cellular therapeutic source due to their minimally invasive surgical approach and remarkable proliferative ability. Aim. This study is the first to investigate the outcomes of the systemic transplantation of DPSCs in a D-galactose (D-gal)-induced rat model of cardiac ageing. Methods. Thirty 9-week-old Sprague-Dawley male rats were randomly assigned into three groups: control, ageing (D-gal), and transplanted groups (D-gal + DPSCs). D-gal (300 mg/kg/day) was administered intraperitoneally daily for 8 weeks. The rats in the transplantation group were intravenously injected with DPSCs at a dose of 1 × 106 once every 2 weeks. Results. The transplanted cells migrated to the heart, differentiated into cardiomyocytes, improved cardiac function, upregulated Sirt1 expression, exerted antioxidative effects, modulated connexin-43 expression, attenuated cardiac histopathological alterations, and had anti-senescent and anti-apoptotic effects. Conclusion. Our results reveal the beneficial effects of DPSC transplantation in a cardiac ageing rat model, suggesting their potential as a viable cell therapy for ageing hearts.
KW - Cardiac aging
KW - D-galactose
KW - Dental pulp stem cell
KW - Rat model
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U2 - 10.7717/peerj.17299
DO - 10.7717/peerj.17299
M3 - Article
AN - SCOPUS:85194180129
SN - 2167-8359
VL - 12
JO - PeerJ
JF - PeerJ
IS - 5
M1 - e17299
ER -
