Deoxycholate-stimulated release of peptide YY from the isolated perfused rabbit left colon

G. H. Ballantyne, W. E. Longo, P. E. Savoca, T. E. Adrian, A. P. Vukasin, A. J. Bilchik, J. Sussman, I. M. Modlin

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The purpose of this study was 1) to measure the effect of graded concentrations of oleic acid and deoxycholic acid (DCA) on the release of peptide YY (PYY) and enteroglucagon and 2) to test whether DCA-stimulated release of PYY was neurally mediated by blocking neuronal conduction with tetrodotoxin. Studies were performed in isolated left colons from New Zealand White rabbits. Oleic acid in concentrations from 0.22 to 22 mM suspended in 10 mM DCA significantly stimulated release of PYY (P < 0.01) but resulted in no graded response (Bartlett's test, P = 0.15). Similarly, oleic acid (2.2 mM) suspended with urosodeoxycholic acid (10 mM) produced no increased release of PYY above that achieved by ursodeoxycholic acid alone. In contrast, oleic acid (2.2 and 22 mM suspended with 10 mM DCA) produced a graded release of enteroglucagon during the stimulated period. Deoxycholic acid caused a concentration-dependent release of PYY (1, 3.3, 10, and 25 mM) during the stimulated period. Deoxycholic acid (1 and 10 mM) did not significantly increase enteroglucagon release. Tetrodotoxin blockade had no effect on release of PYY stimulated by 10 mM DCA. Because PYY and enteroglucagon are both found in colonic endocrine cells, these results suggest that the release of PYY and enteroglucagon are mediated by specific secretagogues and not simply caused by noxious effects of the agonists. Also, this study has demonstrated that DCA-stimulated release of PYY is not dependent on neuronally mediated mechanisms.

Original languageEnglish
Pages (from-to)20/5
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume257
Issue number5
Publication statusPublished - 1989
Externally publishedYes

Keywords

  • Bile acids
  • Deoxycholic acid
  • Enteroglucagon
  • Gastric acid secretion
  • Oleic acid
  • Tetrodotoxin

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Deoxycholate-stimulated release of peptide YY from the isolated perfused rabbit left colon'. Together they form a unique fingerprint.

Cite this