Design and development of novel single multiplex system incorporating 26 rapidly mutating Y-STRs; 26 RM Yplex

Abdulla Albastaki; Mohammed Naji; Reem Lootah; Reem Almheiri; Hanan Almulla; Afra Alreyami; Iman Almarri; Zailiang Yu; Yueke Zhang; Chang Shu; Rashed Alghafri

doi:10.1002/elps.202200099

Design and development of novel single multiplex system incorporating 26 rapidly mutating Y-STRs; 26 RM Yplex

Abdulla Albastaki, Mohammed Naji, Reem Lootah, Reem Almheiri, Hanan Almulla, Afra Alreyami, Iman Almarri, Zailiang Yu, Yueke Zhang, Chang Shu, Rashed Alghafri

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

This article details the development of a single multiplex system amplifying 26 rapidly mutating Y-STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y-STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework.

Original language	English
Pages (from-to)	1911-1919
Number of pages	9
Journal	Electrophoresis
Volume	43
Issue number	18-19
DOIs	https://doi.org/10.1002/elps.202200099
Publication status	Published - Oct 2022
Externally published	Yes

Keywords

STRs
discrimination
multiplex
paternal
rapidly mutating

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry

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10.1002/elps.202200099

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abstract = "This article details the development of a single multiplex system amplifying 26 rapidly mutating Y-STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y-STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework.",

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AU - Albastaki, Abdulla

AU - Naji, Mohammed

AU - Lootah, Reem

AU - Almheiri, Reem

AU - Almulla, Hanan

AU - Alreyami, Afra

AU - Almarri, Iman

AU - Yu, Zailiang

AU - Zhang, Yueke

AU - Shu, Chang

AU - Alghafri, Rashed

PY - 2022/10

Y1 - 2022/10

N2 - This article details the development of a single multiplex system amplifying 26 rapidly mutating Y-STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y-STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework.

AB - This article details the development of a single multiplex system amplifying 26 rapidly mutating Y-STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y-STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework.

KW - STRs

KW - discrimination

KW - multiplex

KW - paternal

KW - rapidly mutating

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JF - Electrophoresis

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Design and development of novel single multiplex system incorporating 26 rapidly mutating Y-STRs; 26 RM Yplex

Abstract

Keywords

ASJC Scopus subject areas

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