@inbook{df8fb9ba4f314898abc3614a78a2c1cd,
title = "Detecting Circadian Rhythms in Human Red Blood Cells by Dielectrophoresis",
abstract = "Dielectrophoresis (DEP) enables the measurement of population-level electrophysiology in many cell types by examining their interaction with an externally applied electric field. Here we describe the application of DEP to the measurement of circadian rhythms in a non-nucleated cell type, the human red blood cell. Using DEP, population-level electrophysiology of ~20,000 red blood cells can be measured from start to finish in less than 3 min, and can be repeated over several days to reveal cell-autonomous daily regulation of membrane electrophysiology. This method is amenable to the characterization of circadian rhythms by altering entrainment and free-run conditions or through pharmacological perturbation.",
keywords = "Circadian rhythm, Dielectrophoresis, Erythrocyte, Red blood cell, TTFL",
author = "Beale, {Andrew D.} and Labeed, {Fatima H.} and Kitcatt, {Stephen J.} and O{\textquoteright}Neill, {John S.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
doi = "10.1007/978-1-0716-2249-0_17",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "255--264",
booktitle = "Methods in Molecular Biology",
}