Detecting Circadian Rhythms in Human Red Blood Cells by Dielectrophoresis

Andrew D. Beale, Fatima H. Labeed, Stephen J. Kitcatt, John S. O’Neill

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)


Dielectrophoresis (DEP) enables the measurement of population-level electrophysiology in many cell types by examining their interaction with an externally applied electric field. Here we describe the application of DEP to the measurement of circadian rhythms in a non-nucleated cell type, the human red blood cell. Using DEP, population-level electrophysiology of ~20,000 red blood cells can be measured from start to finish in less than 3 min, and can be repeated over several days to reveal cell-autonomous daily regulation of membrane electrophysiology. This method is amenable to the characterization of circadian rhythms by altering entrainment and free-run conditions or through pharmacological perturbation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
Publication statusPublished - 2022
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Circadian rhythm
  • Dielectrophoresis
  • Erythrocyte
  • Red blood cell
  • TTFL

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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