TY - JOUR
T1 - Detection and characterization of Salmonella associated with tropical seafood
AU - Shabarinath, S.
AU - Sanath Kumar, H.
AU - Khushiramani, Rekha
AU - Karunasagar, Indrani
AU - Karunasagar, Iddya
N1 - Funding Information:
We gratefully acknowledge the financial support of the Indian Council of Agricultural Research (ICAR), Govt. of India, New Delhi.
PY - 2007/3/10
Y1 - 2007/3/10
N2 - The prevalence of Salmonella in seafood samples collected from the southwest coast of India was studied by conventional culture and by a DNA based molecular technique, polymerase chain reaction (PCR). While conventional culture techniques detected Salmonella in only 20 out of the 100 samples analyzed, direct enrichment lysate PCR detected 52 as positive for Salmonella. A set of three different PCR primers viz., hns, invA and invE were used. It was observed that hns primer detected Salmonella in a significantly higher number of samples. Fourteen out of nineteen isolates belonged to serovar S. enterica Weltevreden. S. Weltevreden isolates were genotyped yielding 4 different patterns both by RAPD and ERIC-PCR but when combined, the overall results discriminated the isolates of S. Weltevreden into 6 different types. This suggests that genetically diverse Salmonella Weltevreden are prevalent in seafood.
AB - The prevalence of Salmonella in seafood samples collected from the southwest coast of India was studied by conventional culture and by a DNA based molecular technique, polymerase chain reaction (PCR). While conventional culture techniques detected Salmonella in only 20 out of the 100 samples analyzed, direct enrichment lysate PCR detected 52 as positive for Salmonella. A set of three different PCR primers viz., hns, invA and invE were used. It was observed that hns primer detected Salmonella in a significantly higher number of samples. Fourteen out of nineteen isolates belonged to serovar S. enterica Weltevreden. S. Weltevreden isolates were genotyped yielding 4 different patterns both by RAPD and ERIC-PCR but when combined, the overall results discriminated the isolates of S. Weltevreden into 6 different types. This suggests that genetically diverse Salmonella Weltevreden are prevalent in seafood.
KW - ERIC
KW - Molecular characterization
KW - PCR
KW - RAPD
KW - RFLP
KW - Salmonella
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U2 - 10.1016/j.ijfoodmicro.2006.09.012
DO - 10.1016/j.ijfoodmicro.2006.09.012
M3 - Article
C2 - 17141346
AN - SCOPUS:33847274099
SN - 0168-1605
VL - 114
SP - 227
EP - 233
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
IS - 2
ER -