Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

Chris Wai Tung Leung; Feng Guo; Yuning Hong; Engui Zhao; Ryan Tsz Kin Kwok; Nelson Lik Ching Leung; Sijie Chen; Nishant N. Vaikath; Omar Mukhtar El-Agnaf; Youhong Tang; Wei Ping Gai; Ben Zhong Tang

doi:10.1039/c4cc07911f

Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

Chris Wai Tung Leung
, Feng Guo
, Yuning Hong
, Engui Zhao
, Ryan Tsz Kin Kwok
, Nelson Lik Ching Leung
, Sijie Chen
, Nishant N. Vaikath
, Omar Mukhtar El-Agnaf
, Youhong Tang
, Wei Ping Gai
, Ben Zhong Tang

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

81 Citations (Scopus)

Abstract

We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.

Original language	English
Pages (from-to)	1866-1869
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	10
DOIs	https://doi.org/10.1039/c4cc07911f
Publication status	Published - Feb 4 2015

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence",

abstract = "We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.",

author = "Leung, \{Chris Wai Tung\} and Feng Guo and Yuning Hong and Engui Zhao and Kwok, \{Ryan Tsz Kin\} and Leung, \{Nelson Lik Ching\} and Sijie Chen and Vaikath, \{Nishant N.\} and El-Agnaf, \{Omar Mukhtar\} and Youhong Tang and Gai, \{Wei Ping\} and Tang, \{Ben Zhong\}",

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doi = "10.1039/c4cc07911f",

language = "English",

volume = "51",

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T1 - Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

AU - Leung, Chris Wai Tung

AU - Guo, Feng

AU - Hong, Yuning

AU - Zhao, Engui

AU - Kwok, Ryan Tsz Kin

AU - Leung, Nelson Lik Ching

AU - Chen, Sijie

AU - Vaikath, Nishant N.

AU - El-Agnaf, Omar Mukhtar

AU - Tang, Youhong

AU - Gai, Wei Ping

AU - Tang, Ben Zhong

PY - 2015/2/4

Y1 - 2015/2/4

N2 - We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.

AB - We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.

UR - https://www.scopus.com/pages/publications/84921506541

UR - https://www.scopus.com/pages/publications/84921506541#tab=citedBy

U2 - 10.1039/c4cc07911f

DO - 10.1039/c4cc07911f

M3 - Article

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AN - SCOPUS:84921506541

SN - 1359-7345

VL - 51

SP - 1866

EP - 1869

JO - Chemical Communications

JF - Chemical Communications

IS - 10

ER -

Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

Abstract

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