Detection of Salmonella Enteritidis in Milk Using Conductometric Immunosensor Coated on Polyaniline/Zinc Oxide Nanocomposite

Sawsan Mutlaq, Borhan Albiss, Anas A. Al-Nabulsi, Tareq Osaili, Tasneem Al-Jaberi, Amin N. Olaimat, Shao Quan Liu, Mutamed M. Ayyash

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The demand for rapid and accurate detection methods for Salmonella Enteritidis necessitates the development of highly sensitive and specific biosensors to ensure proper monitoring of food safety and quality requirements in the food sector and to secure human health. This study focused on development of a polyaniline/zinc oxide (PANI/ZnO) nanocomposite film on a gold electrode conductometric immunosensor for detection of Salmonella Enteritidis. The sensor was modified with monoclonal anti-Salmonella Enteritidis antibodies as biorecognition elements. The fabricated sensor was able to detect and quantify the target pathogen within 30 min and showed a good detection range from 101 to 105 colony-forming units (CFU)/mL for Salmonella Enteritidis and a minimum detection limit of 6.44 CFU/mL in 0.1% peptone water. Additionally, the fabricated sensor showed good selectivity and detection limit toward the target bacterium and successfully determined Salmonella Enteritidis content in ultrahigh heat-treated skim milk samples without pretreatment of the food sample.

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalFoodborne Pathogens and Disease
Volume20
Issue number5
DOIs
Publication statusPublished - May 1 2023

Keywords

  • Salmonella Enteritidis
  • antibody
  • immunosensor
  • milk
  • polyaniline
  • zinc oxide

ASJC Scopus subject areas

  • Microbiology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

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