Detection of the damage caused to DNA by a thiophene-S-oxide using an electrochemical DNA-biosensor

Ana Maria Oliveira Brett, Luis Antônio Da Silva, Hideki Fujii, Shuntaro Mataka, Thies Thiemann

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The electrochemistry of 3,4-dibenzyl-2,5-dimethylthiophene-S-oxide was investigated as well as its interaction with dsDNA. The electrochemical study of the thiophene-S-oxide was performed at different pH values, in a mixed solvent and by solid state voltammetry, and showed that the molecule is reduced at a very negative potential, and the reduction is pH dependent. A glassy carbon electrode modified with a thick thiophene-S-oxide+dsDNA mixture was used to study the interaction of thiophene-S-oxide in situ with dsDNA at a charged interface. The experimental results give strong evidence that the reduced thiophene-S-oxide interacts with dsDNA causing damage with possible strand break and that the thiophene-S-oxide adduct formed with dsDNA can still undergo reduction.

Original languageEnglish
Pages (from-to)91-99
Number of pages9
JournalJournal of Electroanalytical Chemistry
Volume549
Issue numberSUPPL.
DOIs
Publication statusPublished - Jun 5 2003
Externally publishedYes

Keywords

  • 8-Oxoguanine
  • DNA-biosensor
  • DNA-electro chemical biosensors
  • Electrochemistry
  • Oxidation
  • Oxidative damage
  • Reduction
  • Thiophene-S-oxide
  • Voltammetry

ASJC Scopus subject areas

  • Analytical Chemistry
  • General Chemical Engineering
  • Electrochemistry

Fingerprint

Dive into the research topics of 'Detection of the damage caused to DNA by a thiophene-S-oxide using an electrochemical DNA-biosensor'. Together they form a unique fingerprint.

Cite this