Detection of Viruses in Histological Samples

Gulfaraz Khan, Narendran Reguraman

Research output: Chapter in Book/Report/Conference proceedingChapter


Molecular pathology is an integral and essential component of diagnostic pathology, especially in the diagnosis of infectious diseases. Molecular pathology has come a long way from basic histopathological examination of tissues to aid diagnosis. The primary drivers for the emergence of this discipline are the enormous advances in molecular biology and the availability of reagents such as monoclonal antibodies. The application of molecular biology to histology, paved the way for the development of techniques such as in situ hybridization, immunohistochemistry, immunofluorescence, multiplex-staining and PCR. These techniques allow the accurate detection and localization of infectious agents in tissue samples. In this article, we describe these techniques and modifications thereof in the diagnosis of viral infections. We also discuss the advantages, disadvantages, and applicability of these methods in the context of the different types of histological tissues.

Original languageEnglish
Title of host publicationEncyclopedia of Infection and Immunity
Number of pages10
ISBN (Electronic)9780128187319
Publication statusPublished - Jan 1 2022


  • Detection
  • Formalin-fixed tissue
  • Immunohistochemistry
  • In situ hybridization
  • Molecular pathology
  • Multiplex-staining
  • PCR
  • Pathogenesis
  • Viral infection

ASJC Scopus subject areas

  • General Medicine


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