Determination of alkylresorcinols and their metabolites in biological samples by gas chromatography-mass spectrometry

Roksana Wierzbicka, Huaxing Wu, Milan Franek, Afaf Kamal-Eldin, Rikard Landberg

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)

    Abstract

    High throughput GC-MS methods for quantification of alkylresorcinols (AR), biomarkers of whole grain wheat and rye intake, in plasma and adipose tissue and their metabolites in urine were developed and optimised. Alkylresorcinols in plasma (200. μL) and adipose tissues (10-50. mg) were extracted with diethyl ether, whereas main AR metabolites such as DHBA and DHPPA and newly identified metabolites in urine (50. μL) were extracted with ethyl acetate after enzymatic deconjugation. All extracts were purified on OASIS-MAX solid phase extraction cartridges. Plasma and adipose tissue sample extracts were then derivatised with trifluoroacetic anhydride and reconstituted in undecane, whereas AR metabolites in urine samples were derivatised with BSTFA + TMCS (99:1, v/v, 100. μL). Prepared samples were quantified by GC-MS (EI-SIM). Analysis of all compounds in the different matrices showed good selectivity, sensitivity, linearity, precision (<15% within and between batches), adequate recovery (75-108%), and short total run time (10-12. min). The methods developed are applicable to large-scale sample sets such as epidemiological studies.

    Original languageEnglish
    Pages (from-to)120-129
    Number of pages10
    JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
    Volume1000
    DOIs
    Publication statusPublished - Sep 1 2015

    Keywords

    • Alkylresorcinol
    • Alkylresorcinols metabolites
    • Biomarker
    • Gas chromatography-mass spectrometry
    • Whole grain

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Clinical Biochemistry
    • Cell Biology

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