Determination of intrinsic parameters for immobilization reactions of catalase and amyloglucosidase in porous glass supports

Md M. Hossain, D. D. Do

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Experiments have been carried out for immobilizing enzyme‐catalase and amyloglucosidase in controlled‐pore glass particles of two different pore sizes. The experimental results have been analyzed, initial‐stage analysis for the rate parameters of immobilization reactions and long‐time analysis for determining the evolution of the immobilization process. These investigations suggest that the overall process of immobilization is controlled by the restricted diffusion of enzymes into the pores of the support. As a result, immobilized enzyme (IME) can penetrate only up to a certain distance into the support. The penetration depth of IME for the support–enzyme system mentioned have been evaluated from the experimental bulk enzyme concentration data in a batch recirculation reactor.

Original languageEnglish
Pages (from-to)730-736
Number of pages7
JournalBiotechnology and Bioengineering
Volume31
Issue number7
DOIs
Publication statusPublished - May 1988
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Determination of intrinsic parameters for immobilization reactions of catalase and amyloglucosidase in porous glass supports'. Together they form a unique fingerprint.

Cite this