TY - JOUR
T1 - Determination of vancomycin content in dried blood spots for therapeutic drug monitoring
AU - Al-Ghazawi, Mutasim
AU - Daoud, Nour El Huda
AU - Hadidi, Kamal A.
AU - Alzweiri, Muhammed
AU - Aburuz, Salahdein
N1 - Publisher Copyright:
© 2021 by Polish Pharmaceutical Society.
PY - 2021
Y1 - 2021
N2 - Blood spot collection is a simple method to obtain specimens for therapeutic monitoring of drugs, assessing drug adherence, and preventing any potential drug toxicity. A simple chromatographic method for the determination of vancomycin in dried blood spots (DBS) has been developed and validated. Intra and inter-day precisions (measured by CV%) were less than 11.5% and the accuracy deviation was less than 11%. The limits of detection (LOD) and quantification (LOQ) were 1 and 10 ng/mL, respectively. Interestingly, LOQ is smaller than the smallest vancomycin monitoring level. Stability analysis shows that vancomycin is stable for at least three weeks when stored at 4 to 8°C. The high sensitivity of detection and short time of analysis (less than 8 min), combining with the simplicity of the procedure made the method applicable for monitoring vancomycin in dried blood spots, which might be implemented in routine clinical settings.
AB - Blood spot collection is a simple method to obtain specimens for therapeutic monitoring of drugs, assessing drug adherence, and preventing any potential drug toxicity. A simple chromatographic method for the determination of vancomycin in dried blood spots (DBS) has been developed and validated. Intra and inter-day precisions (measured by CV%) were less than 11.5% and the accuracy deviation was less than 11%. The limits of detection (LOD) and quantification (LOQ) were 1 and 10 ng/mL, respectively. Interestingly, LOQ is smaller than the smallest vancomycin monitoring level. Stability analysis shows that vancomycin is stable for at least three weeks when stored at 4 to 8°C. The high sensitivity of detection and short time of analysis (less than 8 min), combining with the simplicity of the procedure made the method applicable for monitoring vancomycin in dried blood spots, which might be implemented in routine clinical settings.
KW - Blood spot
KW - Chromatography
KW - Determination of vancomycin
KW - Therapeutic drug monitoring
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U2 - 10.32383/APPDR/132021
DO - 10.32383/APPDR/132021
M3 - Article
AN - SCOPUS:85105205523
SN - 0001-6837
VL - 78
SP - 3
EP - 10
JO - Acta Poloniae Pharmaceutica - Drug Research
JF - Acta Poloniae Pharmaceutica - Drug Research
IS - 1
ER -