Development of anti-bacterial bio-transfer double sheet layer of polyvinyl alcohol/carboxymethyl cellulose films infused with Astragalus tribuloides leaf extract for beef burgers preservation

This study was conducted to develop biodegradable films using a combination of carboxymethyl cellulose (CMC), polyvinyl alcohol (PVA) and purified leaves extract of Astragalus tribuloides (ATE). Various traits of the films, including their morphology description, thermal behavior, tensile/elongation properties and physical characteristics were examined. The scanning electron microscope (SEM) photographs showed smooth surface with small amounts of ATE, but rougher with higher concentrations of 1.4 %. The Fourier-transform infrared spectroscopy (FTIR) showed a direct relationship between the ATE extract and the PVA/CMC matrix. The films also showed thermal stability behaviors. The study found that the addition of ATE up to 0.8 % caused the films to become opaquer in color and raised their opacity up to 3.909. As a result, the films exhibited reduced moisture absorption (8.21 %) and solubility (27.11 %), making them retard penetrating water vapor up to (1.785 × 10⁻¹⁰ g.m⁻¹ s-1 Pa⁻¹) and could preserve the thiobarbituric acid reactive substances (TBARS) and overall color discrepancies of burger in refrigerated storage.