Development of Cortical Pyramidal Cell and Interneuronal Dendrites: a Role for Kainate Receptor Subunits and NETO1

Alexander Jack, Mohammad I.K. Hamad, Steffen Gonda, Sebastian Gralla, Steffen Pahl, Michael Hollmann, Petra Wahle

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

During neuronal development, AMPA receptors (AMPARs) and NMDA receptors (NMDARs) are important for neuronal differentiation. Kainate receptors (KARs) are closely related to AMPARs and involved in the regulation of cortical network activity. However, their role for neurite growth and differentiation of cortical neurons is unclear. Here, we used KAR agonists and overexpression of selected KAR subunits and their auxiliary neuropilin and tolloid-like proteins, NETOs, to investigate their influence on dendritic growth and network activity in organotypic cultures of rat visual cortex. Kainate at 500 nM enhanced network activity and promoted development of dendrites in layer II/III pyramidal cells, but not interneurons. GluK2 overexpression promoted dendritic growth in pyramidal cells and interneurons. GluK2 transfectants were highly active and acted as drivers for network activity. GluK1 and NETO1 specifically promoted dendritic growth of interneurons. Our study provides new insights for the roles of KARs and NETOs in the morphological and physiological development of the visual cortex.

Original languageEnglish
Pages (from-to)4960-4979
Number of pages20
JournalMolecular Neurobiology
Volume56
Issue number7
DOIs
Publication statusPublished - Jul 1 2019
Externally publishedYes

Keywords

  • Dendritogenesis
  • GluK2
  • Glutamate receptors
  • NETO
  • Postnatal development
  • Rat neocortex

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

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