TY - JOUR
T1 - Development of Cortical Pyramidal Cell and Interneuronal Dendrites
T2 - a Role for Kainate Receptor Subunits and NETO1
AU - Jack, Alexander
AU - Hamad, Mohammad I.K.
AU - Gonda, Steffen
AU - Gralla, Sebastian
AU - Pahl, Steffen
AU - Hollmann, Michael
AU - Wahle, Petra
N1 - Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - During neuronal development, AMPA receptors (AMPARs) and NMDA receptors (NMDARs) are important for neuronal differentiation. Kainate receptors (KARs) are closely related to AMPARs and involved in the regulation of cortical network activity. However, their role for neurite growth and differentiation of cortical neurons is unclear. Here, we used KAR agonists and overexpression of selected KAR subunits and their auxiliary neuropilin and tolloid-like proteins, NETOs, to investigate their influence on dendritic growth and network activity in organotypic cultures of rat visual cortex. Kainate at 500 nM enhanced network activity and promoted development of dendrites in layer II/III pyramidal cells, but not interneurons. GluK2 overexpression promoted dendritic growth in pyramidal cells and interneurons. GluK2 transfectants were highly active and acted as drivers for network activity. GluK1 and NETO1 specifically promoted dendritic growth of interneurons. Our study provides new insights for the roles of KARs and NETOs in the morphological and physiological development of the visual cortex.
AB - During neuronal development, AMPA receptors (AMPARs) and NMDA receptors (NMDARs) are important for neuronal differentiation. Kainate receptors (KARs) are closely related to AMPARs and involved in the regulation of cortical network activity. However, their role for neurite growth and differentiation of cortical neurons is unclear. Here, we used KAR agonists and overexpression of selected KAR subunits and their auxiliary neuropilin and tolloid-like proteins, NETOs, to investigate their influence on dendritic growth and network activity in organotypic cultures of rat visual cortex. Kainate at 500 nM enhanced network activity and promoted development of dendrites in layer II/III pyramidal cells, but not interneurons. GluK2 overexpression promoted dendritic growth in pyramidal cells and interneurons. GluK2 transfectants were highly active and acted as drivers for network activity. GluK1 and NETO1 specifically promoted dendritic growth of interneurons. Our study provides new insights for the roles of KARs and NETOs in the morphological and physiological development of the visual cortex.
KW - Dendritogenesis
KW - GluK2
KW - Glutamate receptors
KW - NETO
KW - Postnatal development
KW - Rat neocortex
UR - http://www.scopus.com/inward/record.url?scp=85056476848&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056476848&partnerID=8YFLogxK
U2 - 10.1007/s12035-018-1414-0
DO - 10.1007/s12035-018-1414-0
M3 - Article
C2 - 30421168
AN - SCOPUS:85056476848
SN - 0893-7648
VL - 56
SP - 4960
EP - 4979
JO - Molecular Neurobiology
JF - Molecular Neurobiology
IS - 7
ER -