Developmental and thyroid hormone-induced expression of preprotemporin genes in the skin of japanese mountain brown frog rana ornativentris

Aya Ohnuma, J. Michael Conlon, Shawichi Iwamuro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

Temporins are a group of small, highly hydrophobic, antimicrobial peptides widely distributed in the skin of frogs from the Ranidae family. In order to examine the mechanisms of regulation of temporin gene expression, we measured expression levels of preprotemporin mRNA in the skin of the Japanese mountain brown frog Rana ornativentris, using a semiquantitative RT-PCR system. Preprotemporin mRNAs were not detected in skin prior to the onset of metamorphosis but their levels increased markedly during metamorphosis, reaching a maximum at the stages of metamorphic climax, suggesting a correlation with thyroid hormone concentrations. Consequently, we examined direct effects of triiodothyronine (T3) on in vivo preprotemporin gene expression. Treatment of adult animals with 2 × 10-9 mol/L T3 for 48 h raised the preprotemporin mRNA levels in skin by 1.5-fold compared with untreated controls.

Original languageEnglish
Title of host publicationTrends in Comparative Endocrinology and Neurobiology
PublisherBlackwell Publishing Inc.
Pages494-496
Number of pages3
ISBN (Print)9781573316712
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1163
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Antimicrobial peptide
  • Frog skin
  • Metamorphosis
  • Temporin, thyroid hormone

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry,Genetics and Molecular Biology
  • History and Philosophy of Science

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