TY - GEN
T1 - Developmental and thyroid hormone-induced expression of preprotemporin genes in the skin of japanese mountain brown frog rana ornativentris
AU - Ohnuma, Aya
AU - Conlon, J. Michael
AU - Iwamuro, Shawichi
PY - 2009/4
Y1 - 2009/4
N2 - Temporins are a group of small, highly hydrophobic, antimicrobial peptides widely distributed in the skin of frogs from the Ranidae family. In order to examine the mechanisms of regulation of temporin gene expression, we measured expression levels of preprotemporin mRNA in the skin of the Japanese mountain brown frog Rana ornativentris, using a semiquantitative RT-PCR system. Preprotemporin mRNAs were not detected in skin prior to the onset of metamorphosis but their levels increased markedly during metamorphosis, reaching a maximum at the stages of metamorphic climax, suggesting a correlation with thyroid hormone concentrations. Consequently, we examined direct effects of triiodothyronine (T3) on in vivo preprotemporin gene expression. Treatment of adult animals with 2 × 10-9 mol/L T3 for 48 h raised the preprotemporin mRNA levels in skin by 1.5-fold compared with untreated controls.
AB - Temporins are a group of small, highly hydrophobic, antimicrobial peptides widely distributed in the skin of frogs from the Ranidae family. In order to examine the mechanisms of regulation of temporin gene expression, we measured expression levels of preprotemporin mRNA in the skin of the Japanese mountain brown frog Rana ornativentris, using a semiquantitative RT-PCR system. Preprotemporin mRNAs were not detected in skin prior to the onset of metamorphosis but their levels increased markedly during metamorphosis, reaching a maximum at the stages of metamorphic climax, suggesting a correlation with thyroid hormone concentrations. Consequently, we examined direct effects of triiodothyronine (T3) on in vivo preprotemporin gene expression. Treatment of adult animals with 2 × 10-9 mol/L T3 for 48 h raised the preprotemporin mRNA levels in skin by 1.5-fold compared with untreated controls.
KW - Antimicrobial peptide
KW - Frog skin
KW - Metamorphosis
KW - Temporin, thyroid hormone
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U2 - 10.1111/j.1749-6632.2008.03665.x
DO - 10.1111/j.1749-6632.2008.03665.x
M3 - Conference contribution
C2 - 19456397
AN - SCOPUS:65449140326
SN - 9781573316712
T3 - Annals of the New York Academy of Sciences
SP - 494
EP - 496
BT - Trends in Comparative Endocrinology and Neurobiology
PB - Blackwell Publishing Inc.
ER -