Dielectrophoretic separation of Bacillus subtilis spores from environmental diesel particles

Henry O. Fatoyinbo, Michael P. Hughes, Stacey P. Martin, Paul Pashby, Fatima H. Labeed

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Isolation of pathogenic bacteria from non-biological material of similar size is a vital sample preparation step in the identification of such organisms, particularly in the context of detecting bio-terrorist attacks. However, many detection methods are impeded by particulate contamination from the environment such as those from engine exhausts. In this paper we use dielectrophoresis - the induced motion of particles in non-uniform fields - to successfully remove over 99% of diesel particulates acquired from environmental samples, whilst letting bacterial spores of B. subtilis pass through the chamber largely unimpeded. We believe that such a device has tremendous potential as a precursor to a range of detection methods, improving the signal-to-noise ratio and ultimately improving detection rates.

Original languageEnglish
Pages (from-to)87-90
Number of pages4
JournalJournal of Environmental Monitoring
Issue number1
Publication statusPublished - 2007
Externally publishedYes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Management, Monitoring, Policy and Law


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