Differential effects of dopamine on a potassium current in cultured rutabaga and wild-type Drosophila cells

Waleed B. Alshuaib; Mini V. Mathew; Mohammed Y. Hasan; Mohamed Abdel Monem Fahim

doi:10.1002/nrc.10072

Differential effects of dopamine on a potassium current in cultured rutabaga and wild-type Drosophila cells

Waleed B. Alshuaib, Mini V. Mathew, Mohammed Y. Hasan, Mohamed Abdel Monem Fahim

Department of Pharmacology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The rutabaga mutation which has a low intracellular cyclic adenosine monophosphate (cAMP) concentration is defective in short-term memory. The aim of this study was to compare modulation effects of dopamine on the delayed-rectifier potassium current (IK_DR) in rutabaga and wild-type Drosophila neurons. The conventional whole-cell patch-clamp technique was applied to cultured Drosophila neurons derived from embryonic neuroblasts. IK_DR was measured from cultured (2 days) wild-type and rutabaga neurons. IK_DR was smaller in rutabaga neurons (373 ± 38 pA) than in wild-type neurons (519 ± 40 pA) but there was no difference in IK_DR activation or inactivation between the two genotypes. We examined the effects of dopamine on IK_DR in wild-type and rutabaga neurons. IK_DR was measured from neurons before and after addition of dopamine to the external solution. Dopamine application reduced IK_DR in wild-type neurons but did not significantly affect IK_DR in rutabaga neurons (single-cell studies). In the presence of dopamine there was no difference in IK_DR between wild-type (344 ± 29 pA) and rutabaga (338 ± 27 pA) neurons (population studies). These results indicate that dopamine differentially affects the delayed-rectifier channel in wild-type and rutabaga neurons. This can alter neuronal excitability in rutabaga and may affect the processing of neural signals necesssary for learning and memory.

Original language	English
Pages (from-to)	175-187
Number of pages	13
Journal	Neuroscience Research Communications
Volume	32
Issue number	3
DOIs	https://doi.org/10.1002/nrc.10072
Publication status	Published - May 2003

Keywords

Dopamine
Learning and memory
Patch-clamp

ASJC Scopus subject areas

Neuroscience(all)

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10.1002/nrc.10072

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title = "Differential effects of dopamine on a potassium current in cultured rutabaga and wild-type Drosophila cells",

abstract = "The rutabaga mutation which has a low intracellular cyclic adenosine monophosphate (cAMP) concentration is defective in short-term memory. The aim of this study was to compare modulation effects of dopamine on the delayed-rectifier potassium current (IKDR) in rutabaga and wild-type Drosophila neurons. The conventional whole-cell patch-clamp technique was applied to cultured Drosophila neurons derived from embryonic neuroblasts. IKDR was measured from cultured (2 days) wild-type and rutabaga neurons. IKDR was smaller in rutabaga neurons (373 ± 38 pA) than in wild-type neurons (519 ± 40 pA) but there was no difference in IKDR activation or inactivation between the two genotypes. We examined the effects of dopamine on IKDR in wild-type and rutabaga neurons. IKDR was measured from neurons before and after addition of dopamine to the external solution. Dopamine application reduced IKDR in wild-type neurons but did not significantly affect IKDR in rutabaga neurons (single-cell studies). In the presence of dopamine there was no difference in IKDR between wild-type (344 ± 29 pA) and rutabaga (338 ± 27 pA) neurons (population studies). These results indicate that dopamine differentially affects the delayed-rectifier channel in wild-type and rutabaga neurons. This can alter neuronal excitability in rutabaga and may affect the processing of neural signals necesssary for learning and memory.",

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Differential effects of dopamine on a potassium current in cultured rutabaga and wild-type Drosophila cells

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