Differentiation-dependent regulation of intestinal vitamin B2 uptake: Studies utilizing human-derived intestinal epithelial Caco-2 cells and native at intestine

Veedamali S. Subramanian, Abhisek Ghosal, Sandeep B. Subramanya, Christian Lytle, Hamid M. Said

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Intestinal epithelial cells undergo differentiation as they move from the crypt to the villi, a process that is associated with up- and downregulation in expression of a variety of genes, including those involved in nutrient absorption. Whether the intestinal uptake process of vitamin B2 [riboflavin (RF)] also undergoes differentiation-dependent regulation and the mechanism through which this occurs are not known. We used humanderived intestinal epithelial Caco-2 cells and native rat intestine as models to address these issues. Caco-2 cells showed a significantly higher carrier-mediated RF uptake in post- than preconfluent cells. This upregulation was associated with a significantly higher level of protein and mRNA expression of the RF transporters hRFVT-1 and hRFVT-3 in the post- than preconfluent cells; it was also accompanied with a significantly higher rate of transcription of the respective genes (SLC52A1 and SLC52A3), as indicated by the higher level of expression of heterogeneous nuclear RNA and higher promoter activity in post- than preconfluent cells. Studies with native rat intestine also showed a significantly higher RF uptake by epithelial cells of the villus tip than epithelial cells of the crypt; this again was accompanied by a significantly higher level of expression of the rat RFVT-1 and RFVT-3 at the protein, mRNA, and heterogeneous nuclear RNA levels. These findings show, for the first time, that the intestinal RF uptake process undergoes differentiation-dependent upregulation and suggest that this is mediated (at least in part) via transcriptional mechanisms.

Original languageEnglish
Pages (from-to)G741-G748
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume304
Issue number8
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Cell differentiation
  • Intestinal transport
  • RFVT-1
  • RFVT-3
  • Riboflavin

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Differentiation-dependent regulation of intestinal vitamin B2 uptake: Studies utilizing human-derived intestinal epithelial Caco-2 cells and native at intestine'. Together they form a unique fingerprint.

Cite this