Abstract
The effect of trypan blue on the 11.5-day rat conceptus after intravitelline Vessel administration is described. For comparison, conceptuses injected with varying volumes of Hanks' BSS have also been studied. Trypan blue significantly retarded the growth and development of conceptuses after 6 hours incubation in vitro. The SEM revealed rounded ectodermal cells, some of which appeared disrupted. These cells seemed to cause some of the intersomitic grooves to disappear, making a number of the somites indistinct from the outside. Unlike cells of uninjected embryos, the surfaces of the affected ectodermal cells lacked microvilli and their perimeters were lined with microvilli-like structures which appeared matted together. It was concluded that trypan blue affected the embryo directly probably by disturbing its fluid and ionic balance.
Original language | English |
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Pages (from-to) | 159-164 |
Number of pages | 6 |
Journal | Acta Anatomica |
Volume | 151 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1994 |
Keywords
- Development
- Rat
- Teratology
- Trypan blue
- Viteline vessel
- Yolk sac
ASJC Scopus subject areas
- Anatomy