Abstract
The concentration and molecular nature of cholecystokinin-like immunoreactivity (CCK-LI) in extracts of porcine intestinal mucosa were determined using sequence-specific radioimmunoassays. Highest CCK concentrations were measured in duodenal mucosa (258 ± 60 pmol/g in the distal duodenum) followed by jejunal mucosa (204 ± 36 pmol/g in the proximal jejunum) and pylorus (51 ± 9 pmol/g). All other gastrointestinal regions proximal to the pylorus and distal to the jejunum contained less than 20 pmol/g. Pancreas contained less than 1 pmol/g. Gel chromatography in 6 M urea revealed four immunoreactive forms and this was confirmed by reverse-phase high-pressure liquid chromatography (HPLC). The predominant molecular form in acid extracts of duodenal mucosa resembled CCK-33 although high concentrations of the larger CCK form ('CCK-58') and of the form intermediate in size between CCK-33 and CCK-8 were measured. A molecular form resembling CCK-8 was the principal form in neutral extracts of the duodenum.
Original language | English |
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Pages (from-to) | 289-298 |
Number of pages | 10 |
Journal | Regulatory Peptides |
Volume | 9 |
Issue number | 4 |
DOIs | |
Publication status | Published - Nov 1984 |
Externally published | Yes |
Keywords
- cholecystokinin
- distribution
- gel chromatography
- heterogeneity
- reverse-phase high-pressure liquid chromatography
- specific radioimmunoassay
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Endocrinology
- Clinical Biochemistry
- Cellular and Molecular Neuroscience