TY - JOUR
T1 - Distribution and postprandial release of porcine peptide YY
AU - Adrian, T. E.
AU - Bacarese-Hamilton, A. J.
AU - Smith, H. A.
AU - Chohan, P.
AU - Manolas, K. J.
AU - Bloom, S. R.
N1 - Funding Information:
This study was supported by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan the Japan Society for the Promotion of Science (JSPS) (KAKENHI grants 23221001, 26241003, and 26701004)
PY - 1987
Y1 - 1987
N2 - Peptide YY (PYY), a thirty-six amino acid intestinal hormonal peptide with a tyrosine residue at each end (hence YY as Y represents tyrosine in the new peptide nomenclature), was found throughout the gastrointestinal tract of the pig. Concentrations were very low in the foregut (antrum, 3.4 ± 0.3 pmol/g; duodenum, 1.1 ± 1.5 pmol/g), higher in the distal small intestine (ileum, 100 ± 13 pmol/g) and very high in the large bowel (descending colon, 270 ± 45 pmol/g). Peptide YY was found to circulate in plasma and concentrations rose substantially in response to eating (fasting, 138 ± 15 pmol/l; postprandial, 263 ± 21 pmol/l; P < 0.001). There was a small but significant portal/arterial gradient in postprandial PYY levels. More than 90% of the immunoreactive PYY in gut extracts eluted, on gel permeation chromatography, in an identical position to pure PYY standard, but small amounts of higher molecular weight material, possibly precursors, were detected. In contrast, plasma from fasting pigs contained a large proportion (60-70%) of these large molecular forms. These findings suggest that the putative pro-PYY may be cleared more slowly from the circulation than the 36 amino acid hormonal peptide. The high concentrations of immunoreactive PYY in the circulation of the young pig may reflect a species difference between pig and man or may indicate an important role for PYY in the developing animal.
AB - Peptide YY (PYY), a thirty-six amino acid intestinal hormonal peptide with a tyrosine residue at each end (hence YY as Y represents tyrosine in the new peptide nomenclature), was found throughout the gastrointestinal tract of the pig. Concentrations were very low in the foregut (antrum, 3.4 ± 0.3 pmol/g; duodenum, 1.1 ± 1.5 pmol/g), higher in the distal small intestine (ileum, 100 ± 13 pmol/g) and very high in the large bowel (descending colon, 270 ± 45 pmol/g). Peptide YY was found to circulate in plasma and concentrations rose substantially in response to eating (fasting, 138 ± 15 pmol/l; postprandial, 263 ± 21 pmol/l; P < 0.001). There was a small but significant portal/arterial gradient in postprandial PYY levels. More than 90% of the immunoreactive PYY in gut extracts eluted, on gel permeation chromatography, in an identical position to pure PYY standard, but small amounts of higher molecular weight material, possibly precursors, were detected. In contrast, plasma from fasting pigs contained a large proportion (60-70%) of these large molecular forms. These findings suggest that the putative pro-PYY may be cleared more slowly from the circulation than the 36 amino acid hormonal peptide. The high concentrations of immunoreactive PYY in the circulation of the young pig may reflect a species difference between pig and man or may indicate an important role for PYY in the developing animal.
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U2 - 10.1677/joe.0.1130011
DO - 10.1677/joe.0.1130011
M3 - Article
C2 - 3585220
AN - SCOPUS:0023127006
SN - 0022-0795
VL - 113
SP - 11
EP - 14
JO - Journal of Endocrinology
JF - Journal of Endocrinology
IS - 1
ER -