Distribution of atrial natriuretic peptide and its effects on contraction and intracellular calcium in ventricular myocytes from streptozotocin-induced diabetic rat

F. C. Howarth; A. Adem; E. A. Adeghate; N. A. Al Ali; A. M. Al Bastaki; F. R. Sorour; R. O. Hammoudi; N. A. Ghaleb; N. J. Chandler; H. Dobrzynski

doi:10.1016/j.peptides.2004.12.003

Distribution of atrial natriuretic peptide and its effects on contraction and intracellular calcium in ventricular myocytes from streptozotocin-induced diabetic rat

F. C. Howarth, A. Adem, E. A. Adeghate, N. A. Al Ali, A. M. Al Bastaki, F. R. Sorour, R. O. Hammoudi, N. A. Ghaleb, N. J. Chandler, H. Dobrzynski

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14 Citations (Scopus)

Abstract

The distribution of atrial natriuretic peptide (ANP) in blood plasma and cardiac muscle and its effects on ventricular myocyte contraction and intracellular free calcium concentration [Ca²⁺]_i in the streptozotocin (STZ)-induced diabetic rat have been investigated. Blood plasma concentration and heart atrial and ventricular contents of ANP were significantly increased in STZ-treated rats compared to age-matched controls. STZ treatment increased the number of ventricular myocytes immunolabeled with antibodies against ANP. In control myocytes the percentage of cells that labeled positively and negatively were 17% versus 83%, respectively. However, in myocytes from STZ-treated rat the percentages were 52% versus 53%. Time to peak (TPK) shortening was significantly and characteristically prolonged in myocytes from STZ-treated rats (360 ± 5 ms) compared to controls (305 ± 5 ms). Amplitude of the Ca²⁺ transient was significantly increased in myocytes from STZ-treated rats compared to controls (0.39 ± 0.02 versus 0.29 ± 0.02 fura-2 RU in controls) and treatment with ANP reduced the amplitude of the Ca²⁺ transient to control levels. ANP may have a protective role in STZ-induced diabetic rat heart.

Original language	English
Pages (from-to)	691-700
Number of pages	10
Journal	Peptides
Volume	26
Issue number	4
DOIs	https://doi.org/10.1016/j.peptides.2004.12.003
Publication status	Published - Apr 2005

Keywords

Atrial natriuretic peptide
Calcium
Diabetes
Ventricular myocytes

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/j.peptides.2004.12.003

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Howarth, F. C., Adem, A., Adeghate, E. A., Al Ali, N. A., Al Bastaki, A. M., Sorour, F. R., Hammoudi, R. O., Ghaleb, N. A., Chandler, N. J., & Dobrzynski, H. (2005). Distribution of atrial natriuretic peptide and its effects on contraction and intracellular calcium in ventricular myocytes from streptozotocin-induced diabetic rat. Peptides, 26(4), 691-700. https://doi.org/10.1016/j.peptides.2004.12.003

Howarth, FC , Adem, A , Adeghate, EA, Al Ali, NA, Al Bastaki, AM, Sorour, FR, Hammoudi, RO, Ghaleb, NA, Chandler, NJ & Dobrzynski, H 2005, 'Distribution of atrial natriuretic peptide and its effects on contraction and intracellular calcium in ventricular myocytes from streptozotocin-induced diabetic rat', Peptides, vol. 26, no. 4, pp. 691-700. https://doi.org/10.1016/j.peptides.2004.12.003

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title = "Distribution of atrial natriuretic peptide and its effects on contraction and intracellular calcium in ventricular myocytes from streptozotocin-induced diabetic rat",

abstract = "The distribution of atrial natriuretic peptide (ANP) in blood plasma and cardiac muscle and its effects on ventricular myocyte contraction and intracellular free calcium concentration [Ca2+]i in the streptozotocin (STZ)-induced diabetic rat have been investigated. Blood plasma concentration and heart atrial and ventricular contents of ANP were significantly increased in STZ-treated rats compared to age-matched controls. STZ treatment increased the number of ventricular myocytes immunolabeled with antibodies against ANP. In control myocytes the percentage of cells that labeled positively and negatively were 17% versus 83%, respectively. However, in myocytes from STZ-treated rat the percentages were 52% versus 53%. Time to peak (TPK) shortening was significantly and characteristically prolonged in myocytes from STZ-treated rats (360 ± 5 ms) compared to controls (305 ± 5 ms). Amplitude of the Ca2+ transient was significantly increased in myocytes from STZ-treated rats compared to controls (0.39 ± 0.02 versus 0.29 ± 0.02 fura-2 RU in controls) and treatment with ANP reduced the amplitude of the Ca2+ transient to control levels. ANP may have a protective role in STZ-induced diabetic rat heart.",

keywords = "Atrial natriuretic peptide, Calcium, Diabetes, Ventricular myocytes",

author = "Howarth, {F. C.} and A. Adem and Adeghate, {E. A.} and {Al Ali}, {N. A.} and {Al Bastaki}, {A. M.} and Sorour, {F. R.} and Hammoudi, {R. O.} and Ghaleb, {N. A.} and Chandler, {N. J.} and H. Dobrzynski",

note = "Funding Information: This study was supported by an Interdisciplinary Grant from the United Arab Emirates University, Al Ain, U.A.E., a travel bursary from the British Council, Abu Dhabi and the British Heart Foundation. The technical support of Anwar Qureshi, Dr. Irwin Chandranath, Dr. Sheela Benedict and Samad Ponery was highly appreciated.",

year = "2005",

month = apr,

doi = "10.1016/j.peptides.2004.12.003",

language = "English",

volume = "26",

pages = "691--700",

journal = "Peptides",

issn = "0196-9781",

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T1 - Distribution of atrial natriuretic peptide and its effects on contraction and intracellular calcium in ventricular myocytes from streptozotocin-induced diabetic rat

AU - Howarth, F. C.

AU - Adem, A.

AU - Adeghate, E. A.

AU - Al Ali, N. A.

AU - Al Bastaki, A. M.

AU - Sorour, F. R.

AU - Hammoudi, R. O.

AU - Ghaleb, N. A.

AU - Chandler, N. J.

AU - Dobrzynski, H.

N1 - Funding Information: This study was supported by an Interdisciplinary Grant from the United Arab Emirates University, Al Ain, U.A.E., a travel bursary from the British Council, Abu Dhabi and the British Heart Foundation. The technical support of Anwar Qureshi, Dr. Irwin Chandranath, Dr. Sheela Benedict and Samad Ponery was highly appreciated.

PY - 2005/4

Y1 - 2005/4

N2 - The distribution of atrial natriuretic peptide (ANP) in blood plasma and cardiac muscle and its effects on ventricular myocyte contraction and intracellular free calcium concentration [Ca2+]i in the streptozotocin (STZ)-induced diabetic rat have been investigated. Blood plasma concentration and heart atrial and ventricular contents of ANP were significantly increased in STZ-treated rats compared to age-matched controls. STZ treatment increased the number of ventricular myocytes immunolabeled with antibodies against ANP. In control myocytes the percentage of cells that labeled positively and negatively were 17% versus 83%, respectively. However, in myocytes from STZ-treated rat the percentages were 52% versus 53%. Time to peak (TPK) shortening was significantly and characteristically prolonged in myocytes from STZ-treated rats (360 ± 5 ms) compared to controls (305 ± 5 ms). Amplitude of the Ca2+ transient was significantly increased in myocytes from STZ-treated rats compared to controls (0.39 ± 0.02 versus 0.29 ± 0.02 fura-2 RU in controls) and treatment with ANP reduced the amplitude of the Ca2+ transient to control levels. ANP may have a protective role in STZ-induced diabetic rat heart.

AB - The distribution of atrial natriuretic peptide (ANP) in blood plasma and cardiac muscle and its effects on ventricular myocyte contraction and intracellular free calcium concentration [Ca2+]i in the streptozotocin (STZ)-induced diabetic rat have been investigated. Blood plasma concentration and heart atrial and ventricular contents of ANP were significantly increased in STZ-treated rats compared to age-matched controls. STZ treatment increased the number of ventricular myocytes immunolabeled with antibodies against ANP. In control myocytes the percentage of cells that labeled positively and negatively were 17% versus 83%, respectively. However, in myocytes from STZ-treated rat the percentages were 52% versus 53%. Time to peak (TPK) shortening was significantly and characteristically prolonged in myocytes from STZ-treated rats (360 ± 5 ms) compared to controls (305 ± 5 ms). Amplitude of the Ca2+ transient was significantly increased in myocytes from STZ-treated rats compared to controls (0.39 ± 0.02 versus 0.29 ± 0.02 fura-2 RU in controls) and treatment with ANP reduced the amplitude of the Ca2+ transient to control levels. ANP may have a protective role in STZ-induced diabetic rat heart.

KW - Atrial natriuretic peptide

KW - Calcium

KW - Diabetes

KW - Ventricular myocytes

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JO - Peptides

JF - Peptides

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ER -

Distribution of atrial natriuretic peptide and its effects on contraction and intracellular calcium in ventricular myocytes from streptozotocin-induced diabetic rat

Abstract

Keywords

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