TY - JOUR
T1 - Distribution of nicotinic cholinergic receptors in the rat tel‐ and diencephalon
T2 - a quantitative receptor autoradiographical study using [3H]‐acetylcholine, [α‐125l]bungarotoxin and [3H]nicotine
AU - HÄRFSTRAND, A.
AU - ADEM, A.
AU - FUXE, K.
AU - AGNATI, L.
AU - ANDERSSON, K.
AU - NORDBERG, A.
PY - 1988/1
Y1 - 1988/1
N2 - The topographical distribution of [α:‐12T]bungarotoxin [125I]BTX, [3H]nicotine ([3H]Nic), [3H]acetylcholine ([3H]ACh) (in the presence of atropine) binding in rat teland diencephalon was investigated using a quantitative receptor autoradiographical technique. With the [3H|ACh and [3H]Nic radioligands, a strong labelling was observed in various thalamic nuclei, including the medial habenula, a moderate labelling in different areas of the cortex cerebri, the nucleus caudatus putamen, the nucleus accumbens and tuberculum olfactorium and a uniform weak labelling in the hypothalamus. When the binding data for [3H]Nic were plotted against binding data for [3H]ACh in various brain nuclei, a significant correlation was obtained. Considering [125I]BTX, the strongest labelling was observed in the lateral mammillary nucleus and the hilus gyrus dentatus of the hippocampal formation. A weak labelling occurred in areas such as the nucleus causatus putamen, the thalamus and the cerebral cortex. No significant correlation was therefore obtained between the degree of [125I]BTX binding in various brain nuclei and the degree of binding observed with [3H]Nic or [3H]ACh. The present results underline the view that the high‐affinity |3H]Nic and [3H]ACh binding sites label the same cholinergic nicotinic receptor binding site, while [125I]BTX labels another subpopulation of nicotinic cholinergic receptors, predominantly found in discrete areas of the hypothalamus and the limbic cortex.
AB - The topographical distribution of [α:‐12T]bungarotoxin [125I]BTX, [3H]nicotine ([3H]Nic), [3H]acetylcholine ([3H]ACh) (in the presence of atropine) binding in rat teland diencephalon was investigated using a quantitative receptor autoradiographical technique. With the [3H|ACh and [3H]Nic radioligands, a strong labelling was observed in various thalamic nuclei, including the medial habenula, a moderate labelling in different areas of the cortex cerebri, the nucleus caudatus putamen, the nucleus accumbens and tuberculum olfactorium and a uniform weak labelling in the hypothalamus. When the binding data for [3H]Nic were plotted against binding data for [3H]ACh in various brain nuclei, a significant correlation was obtained. Considering [125I]BTX, the strongest labelling was observed in the lateral mammillary nucleus and the hilus gyrus dentatus of the hippocampal formation. A weak labelling occurred in areas such as the nucleus causatus putamen, the thalamus and the cerebral cortex. No significant correlation was therefore obtained between the degree of [125I]BTX binding in various brain nuclei and the degree of binding observed with [3H]Nic or [3H]ACh. The present results underline the view that the high‐affinity |3H]Nic and [3H]ACh binding sites label the same cholinergic nicotinic receptor binding site, while [125I]BTX labels another subpopulation of nicotinic cholinergic receptors, predominantly found in discrete areas of the hypothalamus and the limbic cortex.
KW - [3HJacetylcholine
KW - [3H]nicotine
KW - [α‐I]bungarotoxin
KW - brain
KW - nicotinic receptors
KW - quantitative receptor autoradiography
KW - rat
KW - subtypes
UR - http://www.scopus.com/inward/record.url?scp=0023942243&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023942243&partnerID=8YFLogxK
U2 - 10.1111/j.1748-1716.1988.tb08291.x
DO - 10.1111/j.1748-1716.1988.tb08291.x
M3 - Article
C2 - 3223299
AN - SCOPUS:0023942243
SN - 0001-6772
VL - 132
SP - 1
EP - 14
JO - Acta Physiologica Scandinavica
JF - Acta Physiologica Scandinavica
IS - 1
ER -