TY - JOUR
T1 - DNA immunization
T2 - Effect of secretion of DNA-expressed hemagglutinins on antibody responses
AU - Torres, Celia A.T.
AU - Yang, Kejian
AU - Mustafa, Farah
AU - Robinson, Harriet L.
N1 - Funding Information:
We would like to thank H. Drake-Perrow for expert administrative assistance. This work was supported by US National Institutes of Health Research grant RO1 AI 34946 and RO1 AI 35149.
PY - 1999/12/10
Y1 - 1999/12/10
N2 - DNA vaccines expressing plasma membrane and secreted forms of the influenza and measles virus hemagglutinins (HAs) have been used to evaluate the effect of secretion on DNA-raised antibody responses. At low doses of DNA, the plasma membrane form of the influenza virus HA raised higher titers of antibody than the secreted form. The isotype of the DNA-raised antibodies depended on both the method of DNA delivery and the form of the expressed antigen. Following intramuscular injections, DNAs expressing membrane bound forms of the influenza and measles HAs raised predominantly IgG2a. By contrast, DNAs expressing the secreted from of the two HAs as well as another secreted protein, human growth hormone, raised predominantly IgG1. Gene gun delivery resulted in predominantly IgG1 antibody responses for both secreted and membrane bound forms of the hemagglutinins. The raising of predominantly IgG1 by i.m. delivery of the secreted form of the influenza hemagglutinin was IL-4 dependent suggesting that a T-helper 2-biased immune response had been raised. Copyright (C) 1999 Elsevier Science Ltd.
AB - DNA vaccines expressing plasma membrane and secreted forms of the influenza and measles virus hemagglutinins (HAs) have been used to evaluate the effect of secretion on DNA-raised antibody responses. At low doses of DNA, the plasma membrane form of the influenza virus HA raised higher titers of antibody than the secreted form. The isotype of the DNA-raised antibodies depended on both the method of DNA delivery and the form of the expressed antigen. Following intramuscular injections, DNAs expressing membrane bound forms of the influenza and measles HAs raised predominantly IgG2a. By contrast, DNAs expressing the secreted from of the two HAs as well as another secreted protein, human growth hormone, raised predominantly IgG1. Gene gun delivery resulted in predominantly IgG1 antibody responses for both secreted and membrane bound forms of the hemagglutinins. The raising of predominantly IgG1 by i.m. delivery of the secreted form of the influenza hemagglutinin was IL-4 dependent suggesting that a T-helper 2-biased immune response had been raised. Copyright (C) 1999 Elsevier Science Ltd.
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U2 - 10.1016/S0264-410X(99)00345-X
DO - 10.1016/S0264-410X(99)00345-X
M3 - Article
C2 - 10580193
AN - SCOPUS:0032729256
SN - 0264-410X
VL - 18
SP - 805
EP - 814
JO - Vaccine
JF - Vaccine
IS - 9-10
ER -