TY - JOUR
T1 - Draft genomes of Cronobacter sakazakii strains isolated from dried spices bring unique insights into the diversity of plant-associated strains 06 Biological Sciences 0604 Genetics
AU - Jang, Hyein
AU - Woo, Jungha
AU - Lee, Youyoung
AU - Negrete, Flavia
AU - Finkelstein, Samantha
AU - Chase, Hannah R.
AU - Addy, Nicole
AU - Ewing, Laura
AU - Beaubrun, Junia Jean Gilles
AU - Patel, Isha
AU - Gangiredla, Jayanthi
AU - Eshwar, Athmanya
AU - Jaradat, Ziad W.
AU - Seo, Kunho
AU - Shabarinath, Srikumar
AU - Fanning, Séamus
AU - Stephan, Roger
AU - Lehner, Angelika
AU - Tall, Ben D.
AU - Gopinath, Gopal R.
N1 - Funding Information:
We thank the student internship programs sponsored by the Offices of International Affairs of Gachon University, Seongnam-si, Republic of Korea for supporting student interns: Jungha Woo and Youyoung Lee. We thank the University of Maryland at College Park, Joint Institute for Food Safety and Applied Nutrition (JIFSAN) for supporting JIFSAN interns Samantha Finkelstein and Flavia Negrete. We also thank the Oak Ridge Institute for Science and Education of Oak Ridge, Tennessee for sponsoring research fellows Hannah R. Chase, Nicole Addy, and Hyein Jang.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/11/29
Y1 - 2018/11/29
N2 - Cronobacter sakazakii is a Gram-negative opportunistic pathogen that causes life- threatening infantile infections, such as meningitis, septicemia, and necrotizing enterocolitis, as well as pneumonia, septicemia, and urinary tract and wound infections in adults. Here, we report 26 draft genome sequences of C. sakazakii, which were obtained from dried spices from the USA, the Middle East, China, and the Republic of Korea. The average genome size of the C. sakazakii genomes was 4393 kb, with an average of 4055 protein coding genes, and an average genome G + C content of 56.9%. The genomes contained genes related to carbohydrate transport and metabolism, amino acid transport and metabolism, and cell wall/membrane biogenesis. In addition, we identified genes encoding proteins involved in osmotic responses such as DnaJ, Aquaproin Z, ProQ, and TreF, as well as virulence-related and heat shock-related proteins. Interestingly, a metabolic island comprised of a variably-sized xylose utilization operon was found within the spice-associated C. sakazakii genomes, which supports the hypothesis that plants may serve as transmission vectors or alternative hosts for Cronobacter species. The presence of the genes identified in this study can support the remarkable phenotypic traits of C. sakazakii such as the organism's capabilities of adaptation and survival in response to adverse growth environmental conditions (e.g. osmotic and desiccative stresses). Accordingly, the genome analyses provided insights into many aspects of physiology and evolutionary history of this important foodborne pathogen.
AB - Cronobacter sakazakii is a Gram-negative opportunistic pathogen that causes life- threatening infantile infections, such as meningitis, septicemia, and necrotizing enterocolitis, as well as pneumonia, septicemia, and urinary tract and wound infections in adults. Here, we report 26 draft genome sequences of C. sakazakii, which were obtained from dried spices from the USA, the Middle East, China, and the Republic of Korea. The average genome size of the C. sakazakii genomes was 4393 kb, with an average of 4055 protein coding genes, and an average genome G + C content of 56.9%. The genomes contained genes related to carbohydrate transport and metabolism, amino acid transport and metabolism, and cell wall/membrane biogenesis. In addition, we identified genes encoding proteins involved in osmotic responses such as DnaJ, Aquaproin Z, ProQ, and TreF, as well as virulence-related and heat shock-related proteins. Interestingly, a metabolic island comprised of a variably-sized xylose utilization operon was found within the spice-associated C. sakazakii genomes, which supports the hypothesis that plants may serve as transmission vectors or alternative hosts for Cronobacter species. The presence of the genes identified in this study can support the remarkable phenotypic traits of C. sakazakii such as the organism's capabilities of adaptation and survival in response to adverse growth environmental conditions (e.g. osmotic and desiccative stresses). Accordingly, the genome analyses provided insights into many aspects of physiology and evolutionary history of this important foodborne pathogen.
KW - Cronobacter sakazakii
KW - Draft Genomes
KW - Dried Spices
KW - Plant-origin
KW - WGS
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U2 - 10.1186/s40793-018-0339-6
DO - 10.1186/s40793-018-0339-6
M3 - Article
AN - SCOPUS:85057552443
SN - 1944-3277
VL - 13
JO - Standards in Genomic Sciences
JF - Standards in Genomic Sciences
IS - 1
M1 - 35
ER -