TY - JOUR
T1 - Dried blood spot liquid chromatography assay for therapeutic drug monitoring of metformin
AU - AbuRuz, S.
AU - Millership, J.
AU - McElnay, J.
N1 - Funding Information:
The authors wish to acknowledge the Deanship of Academic Research at the University of Jordan for funding this research.
PY - 2006/3/7
Y1 - 2006/3/7
N2 - The use of blood spot collection cards is a simple way to obtain specimens for analysis of drugs for the purpose of therapeutic drug monitoring, assessing adherence to medications and preventing toxicity in routine clinical setting. We describe the development and validation of a microanalytical technique for the determination of metformin from dried blood spots. The method is based on reversed phase high-performance liquid chromatography with ultraviolet detection. Drug recovery in the developed method was found to be more than 84%. The limits of detection and quantification were calculated to be to be 90 and 150 ng/ml, respectively. The intraday and interday precision (measured by CV%) was always less than 9%. The accuracy (measured by relative error, %) was always less than 12%. Stability analysis showed that metformin is stable for at least 2 months when stored at -70°C. The small volume of blood required (10 μL), combined with the simplicity of the analytical technique makes this a useful procedure for monitoring metformin concentrations in routine clinical settings. The method is currently being applied to the analysis of blood spots taken from diabetic patients to assess adherence to medications and relationship between metformin level and metabolic control of diabetes.
AB - The use of blood spot collection cards is a simple way to obtain specimens for analysis of drugs for the purpose of therapeutic drug monitoring, assessing adherence to medications and preventing toxicity in routine clinical setting. We describe the development and validation of a microanalytical technique for the determination of metformin from dried blood spots. The method is based on reversed phase high-performance liquid chromatography with ultraviolet detection. Drug recovery in the developed method was found to be more than 84%. The limits of detection and quantification were calculated to be to be 90 and 150 ng/ml, respectively. The intraday and interday precision (measured by CV%) was always less than 9%. The accuracy (measured by relative error, %) was always less than 12%. Stability analysis showed that metformin is stable for at least 2 months when stored at -70°C. The small volume of blood required (10 μL), combined with the simplicity of the analytical technique makes this a useful procedure for monitoring metformin concentrations in routine clinical settings. The method is currently being applied to the analysis of blood spots taken from diabetic patients to assess adherence to medications and relationship between metformin level and metabolic control of diabetes.
KW - Blood spot
KW - HPLC
KW - Metformin
KW - Therapeutic drug monitoring
UR - http://www.scopus.com/inward/record.url?scp=33644760338&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644760338&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2005.12.050
DO - 10.1016/j.jchromb.2005.12.050
M3 - Article
C2 - 16446128
AN - SCOPUS:33644760338
SN - 1570-0232
VL - 832
SP - 202
EP - 207
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -