TY - JOUR
T1 - Drugs for stroke
T2 - Action of nitrone (Z)-N-(2-bromo-5-hydroxy-4- methoxybenzylidene)-2-methylpropan-2-amine oxide on rat cortical neurons in culture subjected to oxygen-glucose-deprivation
AU - Arce, Carmen
AU - Diaz-Castroverde, Sabela
AU - Canales, María J.
AU - Marco-Contelles, José
AU - Samadi, Abdelouahid
AU - Oset-Gasque, María J.
AU - González, María P.
N1 - Funding Information:
J. Marco-Contelles thanks MICINN (SAF2006-08764-C02-01 and SAF2009-07271), Comunidad de Madrid (S/SAL-0275-2006), for support. M. J. Oset-Gasque thanks MICINN ( SAF2009-11219 , SAF2010-20337 ), ISCIII [Retic RENEVAS ( RD06/0026/012 )], and UCM-Santander ( GR35/10-B ) for financial support. We thank Mikel Erdocia for revising the manuscript.
PY - 2012/9
Y1 - 2012/9
N2 - The action of (Z)-N-(2-bromo-5-hydroxy-4-methoxybenzylidene)-2- methylpropan-2-amine oxide (RP6) on rat cortical neurons in culture, under oxygen-glucose-deprivation conditions, is reported. Cortical neurons in culture were treated during 1 h with OGD. After, they were placed under normal conditions during 24 h (reperfusion) in absence and presence of RP6. Different parameters were measured under each condition (control, 1 h OGD and 1 h OGD + reperfusion in absence and presence of RP6). RP6 protects neurons against ROS generation, lipid peroxidation levels, LDH release and mitochondrial membrane potential alteration, when administered during reperfusion after the OGD damage. Consequently, these results show that nitrone RP6 protects cells against ischemia injury produced during the reoxygenation, and could be a potential drug for the ictus therapy.
AB - The action of (Z)-N-(2-bromo-5-hydroxy-4-methoxybenzylidene)-2- methylpropan-2-amine oxide (RP6) on rat cortical neurons in culture, under oxygen-glucose-deprivation conditions, is reported. Cortical neurons in culture were treated during 1 h with OGD. After, they were placed under normal conditions during 24 h (reperfusion) in absence and presence of RP6. Different parameters were measured under each condition (control, 1 h OGD and 1 h OGD + reperfusion in absence and presence of RP6). RP6 protects neurons against ROS generation, lipid peroxidation levels, LDH release and mitochondrial membrane potential alteration, when administered during reperfusion after the OGD damage. Consequently, these results show that nitrone RP6 protects cells against ischemia injury produced during the reoxygenation, and could be a potential drug for the ictus therapy.
KW - Apoptosis
KW - Cortical neurons
KW - Ischemia
KW - Lipid peroxidation
KW - Necrosis
KW - Neuroprotection
KW - Nitrones
KW - ROS
UR - http://www.scopus.com/inward/record.url?scp=84865789292&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865789292&partnerID=8YFLogxK
U2 - 10.1016/j.ejmech.2012.07.032
DO - 10.1016/j.ejmech.2012.07.032
M3 - Article
C2 - 22858224
AN - SCOPUS:84865789292
SN - 0223-5234
VL - 55
SP - 475
EP - 479
JO - European Journal of Medicinal Chemistry
JF - European Journal of Medicinal Chemistry
ER -