Ecto-5′-nucleotidase-positive cells in the choroid and ciliary body of the rat eye

Adenosine is known to exert multiple functions within the eye. The aim of this report was to find out if adenosine can be produced locally in the choroid and ciliary body. Therefore, I investigated the distribution of ecto-50-nucleotidase (50-NT), the key enzyme for the production of extracellular adenosine. This report provides evidence that 50-NT is expressed in the choroid and in the ciliary body (and its processes) of the rat eye, predominantly in endothelial cells. These locations of 50-NT indicate strategically important production sites of adenosine regulating choroid and ciliary body functions (e.g., blood flow, aqueous fluid production, and immune response).