Ectopic Expression of the Potato StD26 Encoding a Ribosomal Protein S27 Enhances Salt Tolerance in Arabidopsis thaliana

Onoud Alyammahi, Sajeesh Kappachery, Shina Sasi, Ritesh Ghosh, Jelli Venkatesh, Nisha Varghese, Mostafa Abdelrahman, Lam Son Phan Tran, Mayank Anand Gururani

Research output: Contribution to journalArticlepeer-review

Abstract

The potato (Solanum tuberosum) ribosomal protein S27-encoding gene StD26 has previously been linked to hyperosmotic stress tolerance in yeast transformants. Here, we cloned and functionally characterized the StD26 gene in Arabidopsis thaliana to determine its role in salt tolerance in planta. Transgenic Arabidopsis plants ectopically expressing the StD26 exhibited improved phenotypic and physiological responses to 200 mM NaCl, as evidenced by higher shoot and root lengths, higher chlorophyll (Chl) and carotenoid contents, as well as better stomatal conductance, relative to wild-type (WT) plants. Salinity negatively affected photosystem II in WT plants, as indicated by a sharp decrease in Chl a fluorescence kinetics. In contrast, a minimum decrease was observed in transgenic StD26 plants. The transgenic StD26 plants exhibited lower levels of malondialdehyde and electrolyte leakage than WT plants, indicating that the ectopic expression of StD26 gene in Arabidopsis contributes to maintaining cell membrane integrity in transgenic plants under salinity. Ectopic expression of the StD26 gene enhanced the expression of numerous antioxidant- and stress-related genes, providing strong evidence that transgenic plants were better adapted to salinity conditions compared with WT plants. Our findings provide a role of the StD26 gene and contribute to our knowledge on the complexity of salt tolerance mechanisms in plants.

Original languageEnglish
JournalJournal of Plant Growth Regulation
DOIs
Publication statusAccepted/In press - 2023

Keywords

  • Antioxidant
  • Arabidopsis thaliana
  • Ribosomal protein S27
  • Salt stress
  • Transgenic analysis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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